TRIS hydrochloride
TRIS hydrochloride (CAS: 1185-53-1), with the molecular formula NH2C(CH2OH)3 · HCl and a molecular weight of 157.60, is a vital biological buffer supplied by TSS. This highly pure, white powder is essential for maintaining stable pH in various biochemical and cell culture applications. Its ELITE quality and suitability for electrophoresis make it a reliable choice for demanding laboratory research and pharmaceutical development.
- IUPAC
- TRIS HCl,TRIS hydrochloride,Tris(hydroxymethyl)aminomethane hydrochloride
- Synonyms
- TRIS HClTris(hydroxymethyl)aminomethane hydrochloride
Biological Buffering
TRIS hydrochloride is widely used to prepare buffer solutions, particularly in the pH range of 7.0-9.0. It is a key component in electrophoresis buffers for nucleic acid and protein separation due to its low UV absorbance at 260 and 280 nm.
Cell Culture Media
This compound is tested for suitability in cell culture, ensuring it supports cellular viability and function. Its buffering capacity is crucial for maintaining the stable physiological environment required for cell growth and experiments.
Biochemical Assays
As a high-purity reagent, TRIS hydrochloride is employed in a variety of biochemical assays and enzymatic studies where precise pH control is critical for enzyme activity and reaction kinetics.
| Molecular weight | 157.60 |
|---|---|
| Linear formula | NH2C(CH2OH)3 · HCl |
| Assay | ≥99.0% (titration) |
| Grade | Non-Compendial |
| Form | powder |
| Solubility | H2O: soluble667 mg/mL |
| pH | 2.5-4.0 (25 °C, 4 M in H2O) |
| Melting point | 150-152 °C |
| Loss on drying | ≤0.2% loss on drying, 110 °C |
| Impurities tested | DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detectedendotoxin and total aerobic microbial count, tested≤0.5% water (Karl Fischer) |
| Suitability | cell culture testedsuitable for electrophoresis |
| Biological source | from synthetic |
| Water hazard class (WGK, DE) | 1 |
|---|---|
| Cation traces | heavy metals (as Pb): ≤5 ppm |
| Anion traces | sulfate (SO42-): ≤50 mg/kg |
| Ignition residue | ≤0.2% (as SO4) |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Quality Level | ELITE |
|---|---|
| Product Line | PharmaGrade |
| Foreign Activity | Cytotoxicity, DNase, NICKase, RNase, and Protease; tested |
| Uv Absorption | λ: 260 nm Amax: 0.12λ: 280 nm Amax: 0.08 |
| Absorption | A40%/290 ≤0.05 |
| Useful Ph Range | 7.0 - 9.0 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is TRIS hydrochloride used for?
+
TRIS hydrochloride is primarily used as a biological buffer, particularly in electrophoresis and cell culture applications, due to its ability to maintain stable pH in the 7.0-9.0 range.
What is the CAS number and molecular formula for TRIS hydrochloride?
+
The CAS number is 1185-53-1, and the molecular formula is NH2C(CH2OH)3 · HCl.
What grade and purity does Tech Serve Solutions supply for TRIS hydrochloride?
+
Tech Serve Solutions supplies TRIS hydrochloride at an assay of ≥99.0% (titration), meeting the ELITE quality level and PharmaGrade product line. It is graded as Non-Compendial.
What are the safety considerations when handling TRIS hydrochloride?
+
TRIS hydrochloride is classified as a WGK 1 substance in Germany. It is important to handle it with appropriate personal protective equipment to avoid irritation. Consult the Safety Data Sheet for detailed handling and hazard information.
How is TRIS hydrochloride packed and shipped by TSS?
+
TRIS hydrochloride is exported globally by TSS. Specific packaging details are available upon request to ensure safe and compliant transport.
How can I request a quote or sample of TRIS hydrochloride?
+
To request a quote or sample of TRIS hydrochloride, please contact the TSS sales team directly through our website or listed contact information.
▶ Related products
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (C8H13N3O4) — chemical structure, CAS 138472-01-2; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F138472-01-2.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
C8H13N3O4
Biochemicals & Reagents![(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide (C14H18N4O4) — chemical structure, CAS 162626-99-5; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F162626-99-5.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide
C14H18N4O4
Biochemicals & Reagents
(±)-2,5-Dimethoxy-4-bromoamphetamine hydrobromide
Biochemicals & Reagents
(±)-2-(p-Methoxyphenoxy)propionic acid
C10H12O4
Biochemicals & Reagents
(±)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide
C10H13NO2·HBr
Biochemicals & Reagents
(±)-2-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxyoctanoic acid
C8H16O3
Biochemicals & Reagents▶ Explore more
3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis
3-Methyl-1,5-pentanediol
Chemical Synthesis
3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis