Pyroglutamic acid p-nitroanilide
Pyroglutamic acid p-nitroanilide (CAS: 66642-35-1), with the molecular formula C11H11N3O4 and a molecular weight of 249.22 g/mol, is a chemical compound primarily utilised as an enzyme substrate. Its specific structure makes it valuable in biochemical research and diagnostics for studying enzymatic activities. Stored at -20°C, this reagent is a key component in various assays within the life sciences sector, supporting investigations into enzyme kinetics and function.

Enzyme Substrate
Pyroglutamic acid p-nitroanilide serves as a chromogenic substrate for enzymes, particularly those that cleave pyroglutamic acid residues. Its hydrolysis releases a p-nitroaniline chromophore, allowing for spectrophotometric detection and quantification of enzyme activity.
Biochemical Research
This compound is employed in biochemical research to characterise and identify enzyme functions. It aids in understanding enzyme mechanisms, screening inhibitors, and developing diagnostic assays that rely on enzymatic reactions.
Diagnostic Assays
In the development of diagnostic tools, Pyroglutamic acid p-nitroanilide can be integrated into assays for detecting specific enzyme levels in biological samples. This facilitates the monitoring of various physiological or pathological states.
| Molecular weight | 249.22 |
|---|---|
| Empirical formula | C11H11N3O4 |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Pyroglutamic acid p-nitroanilide used for?
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Pyroglutamic acid p-nitroanilide is used as a chromogenic substrate in biochemical research and diagnostic assays to study enzyme activity.
What are the CAS number and formula for Pyroglutamic acid p-nitroanilide?
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The CAS number is 66642-35-1, and the empirical formula is C11H11N3O4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Pyroglutamic acid p-nitroanilide as a reagent for research purposes. The specific assay and purity are detailed in the product specifications. TSS does not represent this product as USP, BP, or EP grade.
How should Pyroglutamic acid p-nitroanilide be handled and stored?
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Handle Pyroglutamic acid p-nitroanilide with appropriate personal protective equipment, including eyeshields, gloves, and an N95 or P1 respirator filter. Store the chemical at -20°C.
Is Pyroglutamic acid p-nitroanilide a controlled substance?
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Based on the provided data, there is no indication that Pyroglutamic acid p-nitroanilide is a controlled substance.
How is this product packed and exported?
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Tech Serve Solutions, a global supplier and exporter, packs and exports chemicals according to international shipping regulations to ensure safe and compliant delivery worldwide.
How can I request a sample or quote?
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To request a sample or quote for Pyroglutamic acid p-nitroanilide, please contact the Tech Serve Solutions sales team through our website or designated contact channels.
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