p-Nitrophenyl Phosphate Liquid Substrate System
p-Nitrophenyl Phosphate Liquid Substrate System (CAS: 4264-83-9, Formula: O2NC6H4OP(O)(ONa)2, MW: 263.05) is a liquid biochemical reagent utilized as a sensitive substrate for alkaline phosphatase. Upon enzymatic cleavage, it produces a soluble yellow product, measurable at 405 nm, which can be quantified after stopping the reaction with 3N NaOH. This substrate system is specifically recommended for ELISA and other microwell-based colorimetric assays.
- IUPAC
- 4-Nitrophenyl phosphate disodium salt solution,pNPP
- Synonyms
- 4-Nitrophenyl phosphate disodium salt solutionpNPP
Enzyme Substrate for Alkaline Phosphatase
This liquid substrate system is designed for the quantitative determination of alkaline phosphatase activity. It provides a colorimetric readout based on the release of p-nitrophenol.
ELISA Applications
It is well-suited for enzyme-linked immunosorbent assays (ELISA), particularly in microwell formats, where its soluble end product can be accurately measured spectrophotometrically.
Colorimetric Detection Assays
The system facilitates colorimetric detection in various biochemical assays where alkaline phosphatase is used as a detection enzyme, offering a convenient and stable liquid format.
| Molecular weight | 263.05 |
|---|---|
| Linear formula | O2NC6H4OP(O)(ONa)2 |
| Form | liquid |
| Storage temperature | −20°C |
| Application | Substrate for alkaline phosphatase that yields a soluble yellow end product that may be read at 405 nm. The reaction may be stopped with 3N NaOH and read at 405 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. |
Hazard statements
- H315Causes skin irritation
- H318Causes serious eye damage
- H373May cause damage to organs through prolonged exposure
Precautionary statements
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
| Water hazard class (WGK, DE) | 3 |
|---|---|
| Hazard codes (EU) | Xn |
| Risk statements (R) | 21/22-38-41-48/22 |
| Safety statements (S) | 26-36/37/39 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Shipped In | wet ice |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is p-Nitrophenyl Phosphate Liquid Substrate System used for?
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It is used as a substrate for alkaline phosphatase in biochemical assays, particularly in ELISA and other microwell procedures, yielding a yellow soluble product measurable at 405 nm.
What is the CAS number and chemical formula?
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The CAS number is 4264-83-9 and the linear formula is O2NC6H4OP(O)(ONa)2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product as a liquid substrate system for research and laboratory use. The specific assay and purity are detailed in the product specifications; it is not supplied as USP, BP, or EP grade unless explicitly stated.
How should this product be handled and stored?
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This product is classified as Danger, causing skin irritation, serious eye damage, and may cause damage to organs through prolonged or repeated exposure (H315-H318-H373). Handle with appropriate personal protective equipment, including gloves and eye protection. Store at -20°C, shipped on wet ice.
How is this product shipped?
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This product is shipped on wet ice to maintain its stability during transit.
How can I request a quote or sample?
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Please contact our sales team or use the online enquiry form to request a quote or a sample of p-Nitrophenyl Phosphate Liquid Substrate System.
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