pGlu-Phe-Leu p-nitroanilide
pGlu-Phe-Leu p-nitroanilide (CAS 85901-57-1) is a synthetic chromogenic substrate with the molecular formula C26H31N5O6 and a molecular weight of 509.55 g/mol. This compound is primarily utilised in biochemical research for the detection and quantification of enzyme activity. Its structure allows for the release of p-nitroaniline upon enzymatic cleavage, enabling spectrophotometric measurement.
Enzyme Activity Assays
This compound serves as a chromogenic substrate for the study of enzymes, particularly proteases. Upon enzymatic hydrolysis, it releases p-nitroaniline, a coloured product that can be quantified spectrophotometrically to determine enzyme kinetics and activity levels.
Biochemical Research Reagent
pGlu-Phe-Leu p-nitroanilide is employed in various biochemical research applications. It aids in the characterisation of enzyme function and the screening of enzyme inhibitors within experimental settings.
Fluorogenic and Chromogenic Studies
As a substrate that yields a detectable signal upon reaction, it is valuable in both fluorogenic and chromogenic assay development. This facilitates sensitive detection methods in biological sample analysis.
| Molecular weight | 509.55 |
|---|---|
| Empirical formula | C26H31N5O6 |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is pGlu-Phe-Leu p-nitroanilide used for?
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pGlu-Phe-Leu p-nitroanilide is used as a chromogenic substrate in biochemical research for studying enzyme activity, particularly that of proteases. Enzymatic cleavage releases p-nitroaniline, which can be measured spectrophotometrically.
What is the CAS number and formula for pGlu-Phe-Leu p-nitroanilide?
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The CAS number for pGlu-Phe-Leu p-nitroanilide is 85901-57-1, and its molecular formula is C26H31N5O6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product for research and laboratory use. Purity and specific grade details are available upon request or via product specification sheets. TSS does not represent this product as USP, BP, EP, or other pharmacopoeial grades.
How should pGlu-Phe-Leu p-nitroanilide be handled and stored?
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Handle pGlu-Phe-Leu p-nitroanilide with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or P1 (EN143) respirator filter is recommended. Store the chemical at -20°C.
Does Tech Serve Solutions export pGlu-Phe-Leu p-nitroanilide?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including pGlu-Phe-Leu p-nitroanilide, and can fulfil export orders worldwide.
How can I request a sample or quote for pGlu-Phe-Leu p-nitroanilide?
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To request a sample or a quotation, please contact our sales team through the website or by phone, providing the product name and CAS number (85901-57-1).
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