Eosin 5-isothiocyanate
Eosin 5-isothiocyanate (CAS: 60520-47-0), with the molecular formula C21H7Br4NO5S and a molecular weight of 704.96 g/mol, is a specialised fluorescent probe. It is primarily utilised in biochemical research and diagnostic assays. Its reactive isothiocyanate group allows for covalent attachment to molecules bearing primary amine groups, enabling applications in fluorescence-based detection and FRET assays when paired with fluorescein-5′-isothiocyanate (FITC).
- IUPAC
- EITC
Fluorescence Resonance Energy Transfer (FRET) Assays
Eosin 5-isothiocyanate (EITC) can be employed alongside fluorescein-5′-isothiocyanate (FITC) labelled molecules in FRET-based assays. This allows for the study of molecular interactions and conformational changes.
Biochemical Labelling
The isothiocyanate group on EITC readily reacts with primary amines on biomolecules such as proteins and peptides. This enables fluorescent labelling for various detection and imaging applications.
Fluorescent Probes in Microscopy
As a fluorescent probe, EITC can be used to tag cellular components or specific molecules for visualisation and analysis using fluorescence microscopy techniques.
| Molecular weight | 704.96 |
|---|---|
| Empirical formula | C21H7Br4NO5S |
| Assay | ≥95.0% (UV) |
| Storage temperature | 2-8°C |
| Application | Eosin 5-isothiocyanate (EITC) is a fluorescence probe. EITC tagged molecules may be used together with fluorescein-5′-isothiocyanate (FITC) tagged molecules in fluoresce resonance energy transfer (FRET) based assays. |
Hazard statements
- H317May cause an allergic skin reaction
- H334May cause allergy or asthma symptoms if inhaled
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P280Wear protective gloves, clothing and eye/face protection
- P342
| Protective equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 42 |
| Safety statements (S) | 22-24/25 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 521; λem 544 (pH 9.0) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Eosin 5-isothiocyanate used for?
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Eosin 5-isothiocyanate (EITC) is used as a fluorescence probe in biochemical research and diagnostic assays. It is particularly useful in Fluorescent Resonance Energy Transfer (FRET) based assays when used in conjunction with fluorescein-5′-isothiocyanate (FITC) labelled molecules.
What are the CAS number and molecular formula for Eosin 5-isothiocyanate?
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The CAS number for Eosin 5-isothiocyanate is 60520-47-0, and its molecular formula is C21H7Br4NO5S.
What grade and purity does Tech Serve Solutions supply for Eosin 5-isothiocyanate?
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Tech Serve Solutions supplies Eosin 5-isothiocyanate with an assay of ≥95.0% (UV). TSS does not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
How should Eosin 5-isothiocyanate be handled safely?
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Eosin 5-isothiocyanate is classified with Danger signal word, with hazard statements H317 (May cause an allergic skin reaction) and H334 (May cause allergy or asthma symptoms or breathing difficulties if inhaled). Appropriate personal protective equipment, including a dust mask type N95 (US), eyeshields, faceshields, and gloves, is required. Handle in a well-ventilated area or under a fume hood.
How is Eosin 5-isothiocyanate packed and exported?
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Eosin 5-isothiocyanate is typically stored at 2-8°C. TSS is a specialist global supplier and exporter of fine chemicals and reagents.
How can I request a quote or sample for Eosin 5-isothiocyanate?
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To request a quote or sample for Eosin 5-isothiocyanate, please contact our sales department through the TSS website or by phone.
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