Nitrazine Yellow
Nitrazine Yellow (CAS: 5423-07-4), a disodium salt with the molecular formula C16H8N4Na2O11S2 and a molecular weight of 542.36 g/mol, is a synthetic organic compound. It functions as a pH indicator, exhibiting a distinct colour change over a specific pH range. Its primary applications are found in biological staining and as a reagent in laboratory settings, particularly within cell biology and histology.
- IUPAC
- 2-(2,4-Dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt,2-(2,4-Dinitrophenylazo)naphthol-3,6-disulfonic acid disodium salt,Nitrazol Yellow
- Synonyms
- Nitrazol Yellow2-(2,4-Dinitrophenylazo)-1-hydroxynaphthalene-3,6-disulfonic acid disodium salt2-(2,4-Dinitrophenylazo)naphthol-3,6-disulfonic acid disodium salt

pH Indicator
Nitrazine Yellow is widely used as a pH indicator in various laboratory procedures. It undergoes a colour transition, typically changing from yellow in acidic solutions to blue in alkaline conditions, allowing for visual pH determination.
Biological Staining
This compound serves as a stain in cell biology and histology. Its ability to selectively colour cellular components aids in microscopic examination and tissue analysis, contributing to research and diagnostic applications.
Diagnostic Reagent
In specific diagnostic tests, Nitrazine Yellow can be employed as a reagent for assessing pH levels in biological samples. Its clear colour change provides a straightforward method for qualitative analysis.
| Molecular weight | 542.36 |
|---|---|
| Empirical formula | C16H8N4Na2O11S2 |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Risk statements (R) | 5 |
| Safety statements (S) | 15-22-24/25 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 14890 |
|---|---|
| Absorption | λmax 586 nm |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Nitrazine Yellow used for?
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Nitrazine Yellow is primarily used as a pH indicator and as a stain in cell biology and histology.
What are the CAS number and chemical formula for Nitrazine Yellow?
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The CAS number for Nitrazine Yellow is 5423-07-4, and its chemical formula is C16H8N4Na2O11S2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Nitrazine Yellow in a technical/research grade. We do not represent it as USP, BP, or EP grade.
What are the safety precautions for handling Nitrazine Yellow?
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When handling Nitrazine Yellow, it is essential to wear eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used. The substance has a WGK rating of 3 in Germany, indicating it is hazardous to water.
How is Nitrazine Yellow packed, shipped, and exported?
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Nitrazine Yellow is carefully packed to ensure integrity during transit and is available for domestic and international export by Tech Serve Solutions.
How can I request a sample or quote for Nitrazine Yellow?
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To request a sample or a quote for Nitrazine Yellow, please contact our sales team through the website or by phone, providing your specific requirements.
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