Nessler’s reagent
Nessler’s reagent (CAS 7783-33-7, K2HgI4) is a solution primarily used for the detection and quantification of ammonia. This laboratory reagent, also known as dipotassium tetraiodomercurate(II) in dilute sodium hydroxide, is crucial in analytical chemistry for determining ammonia concentrations in water and other matrices. Its effectiveness makes it indispensable for environmental testing and various industrial quality control processes.
- IUPAC
- Dipotassium tetraiodomercurate(II) in dilute sodium hydroxide

Ammonia Analysis
Nessler's reagent is the standard analytical reagent for determining ammonia levels. It reacts with ammonia to form a characteristic yellow to brown coloration, the intensity of which is proportional to the ammonia concentration, making it suitable for colorimetric analysis.
Water Quality Testing
It is widely employed in the testing of potable and wastewater to monitor ammonia contamination, a key indicator of pollution and potential health risks. Accurate determination of ammonia is vital for regulatory compliance and public health.
Spectrophotometry
The reagent is used in UV/Visible (UV/VIS) spectroscopy for quantitative analysis of ammonia. The coloured complex formed allows for precise measurement of absorbance at specific wavelengths.
| Molecular weight | 786.40 |
|---|---|
| Empirical formula | K2HgI4 |
| Density | 1.097 g/mL at 25 °C(lit.) |
| Application | For ammonium analysis. Allow to settle and use the clear supernatant liquid |




Hazard statements
- H301Toxic if swallowed
- H314Causes severe skin burns and eye damage
- H341Suspected of causing genetic defects
- H373May cause damage to organs through prolonged exposure
- H410Very toxic to aquatic life with long-lasting effects
Precautionary statements
- P273Avoid release to the environment
- P280Wear protective gloves, clothing and eye/face protection
- P301IF SWALLOWED
- P305IF IN EYES
- P310Immediately call a POISON CENTER or doctor
- P501Dispose of contents and container per local regulations
| Protective equipment | Faceshields, full-face respirator (US), Gloves, Goggles, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter |
|---|---|
| Transport (UN / ADR) | UN 2922 8/PG 2 |
| Water hazard class (WGK, DE) | 2 |
| Hazard codes (EU) | T,N |
| Risk statements (R) | 25-35-48/21/22-50/53-68 |
| Safety statements (S) | 26-36/37/39-45-61 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Nessler’s reagent used for?
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Nessler’s reagent is used for the detection and quantitative determination of ammonia, particularly in water analysis.
What is the CAS number and chemical formula for Nessler’s reagent?
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The CAS number is 7783-33-7, and the chemical formula is K2HgI4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Nessler’s reagent typically as a laboratory or technical grade. The precise assay is detailed on the product's Certificate of Analysis; we do not represent this product as USP, BP, or EP grade.
How should Nessler’s reagent be handled safely?
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Nessler's reagent is a corrosive and toxic substance. Handle with extreme care, using appropriate personal protective equipment including gloves, goggles, face shields, and a suitable respirator. Avoid contact with skin and eyes, and ensure adequate ventilation. Refer to the Safety Data Sheet (SDS) for comprehensive handling information.
How is Nessler’s reagent packed and shipped?
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Nessler’s reagent is carefully packed according to international shipping regulations for hazardous materials and exported globally by TSS.
How can I request a sample or quote for Nessler’s reagent?
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To request a sample or a quote, please contact our sales team through the website or by phone, providing your specific requirements and shipping details.
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