N-p-Tosyl-L-phenylalanine chloromethyl ketone

CAS 402-71-1C17H18ClNO3SBiochemicals & Reagents

N-p-Tosyl-L-phenylalanine chloromethyl ketone (CAS 402-71-1) is a white powder with a molecular weight of 351.85 g/mol. This compound serves as an irreversible inhibitor for certain serine proteases. It is particularly useful for inhibiting chymotrypsin in the preparation of trypsin, finding application in biochemical research and enzyme studies. Supplied by TSS, it meets a purity standard of ≥97% by TLC.

IUPAC: (S)-1-Chloro-3-tosylamido-4-phenyl-2-butanone,(S)-1-Chloro-4-phenyl-3-tosylamido-2-butanone,TPCK,Tosyl-L-phenylalanyl-chloromethane
Synonyms: (S)-1-Chloro-3-tosylamido-4-phenyl-2-butanone(S)-1-Chloro-4-phenyl-3-tosylamido-2-butanoneTPCKTosyl-L-phenylalanyl-chloromethane

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N-p-Tosyl-L-phenylalanine chloromethyl ketone (C17H18ClNO3S) — chemical structure, CAS 402-71-1; Life Science, fine chemical supplied by Tech Serve Solutions

A to CBioactive Small MoleculesBiochemicals and ReagentsBromelainCell BiologyChymotrypsinalpha-Enzyme InhibitorsEnzyme Inhibitors by EnzymeEnzymes

01 /Applications

Serine Protease Inhibition

N-p-Tosyl-L-phenylalanine chloromethyl ketone acts as an irreversible inhibitor of specific serine proteases. It is widely employed in biochemical research to control enzymatic activity.

Chymotrypsin Inhibition in Trypsin Preparations

This compound is specifically utilised to inhibit chymotrypsin activity within trypsin preparations. This ensures the purity and specificity of trypsin for downstream applications in protein research and biochemistry.

Biochemical Research Tool

As a selective enzyme inhibitor, it serves as a valuable tool in various biochemical and cell biology studies. Researchers utilise it to investigate protease functions and signalling pathways.

02 /Properties

Molecular weight	351.85
Empirical formula	C17H18ClNO3S
Assay	≥97% (TLC)
Form	powder
Colour	white
Solubility	DMSO: >10 mg/mL (stable for several months at 4°C.)ethanol: soluble20 mg/mL (stable for several months at 4°C. Stock solutions (10 mM in methanol or ethanol) are stable for several months at 4°C.)
Storage temperature	−20°C
Melting point	106-108 °C(lit.)
Application	Irreversible inhibitor of certain serine proteases; used to inhibit chymotrypsin in preparations of trypsin.

03 /Safety & handling

GHS hazard pictogram: Corrosive (GHS05) — Corrosive

GHS hazard pictogram: Harmful / irritant (GHS07) — Harmful / irritant

Danger

Hazard statements

H315Causes skin irritation
H318Causes serious eye damage
H335May cause respiratory irritation

Precautionary statements

P261Avoid breathing dust, fume, gas or vapours
P280Wear protective gloves, clothing and eye/face protection
P305IF IN EYES

Protective equipment	dust mask type N95 (US), Eyeshields, Gloves
Transport (UN / ADR)	UN 3335
Water hazard class (WGK, DE)	3
Hazard codes (EU)	Xi
Risk statements (R)	37/38-41
Safety statements (S)	26-36

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

402-71-1

MDL number

MFCD00000935

PubChem substance

24278737

Beilstein registry

2895215

05 /Additional specifications

Gene Information	human ... NFKB1(4790), NKAP(79576), NKRF(55922), NOS2(4843), PTGS2(5743)
Potency	10-100 μM effective concentration

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is N-p-Tosyl-L-phenylalanine chloromethyl ketone used for?

N-p-Tosyl-L-phenylalanine chloromethyl ketone is used as an irreversible inhibitor of certain serine proteases, particularly for inhibiting chymotrypsin in trypsin preparations, and as a tool in biochemical research.

What are the CAS number and formula for N-p-Tosyl-L-phenylalanine chloromethyl ketone?

The CAS number is 402-71-1. The empirical formula is C17H18ClNO3S.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies N-p-Tosyl-L-phenylalanine chloromethyl ketone with a minimum purity of ≥97% (TLC). TSS does not represent this product as USP, BP, EP, or any pharmacopoeia grade.

What are the safety and handling considerations for this chemical?

This compound is classified under GHS05 and GHS07 with the signal word 'Danger'. It carries hazard statements H315 (Causes skin irritation), H318 (Causes serious eye damage), and H335 (May cause respiratory irritation). Appropriate PPE including a dust mask type N95, eyeshields, and gloves should be used. Handle in a well-ventilated area.

How is N-p-Tosyl-L-phenylalanine chloromethyl ketone packed and shipped?

N-p-Tosyl-L-phenylalanine chloromethyl ketone is typically supplied as a white powder and stored at -20°C. Specific packaging and shipping details for export can be provided upon request.

How can I request a quote or sample?

To request a quote or a sample of N-p-Tosyl-L-phenylalanine chloromethyl ketone, please contact the TSS sales team via our website or direct inquiry channels.