N-Benzoyl-Val-Gly-Arg p-nitroanilide hydrochloride
N-Benzoyl-Val-Gly-Arg p-nitroanilide hydrochloride (CAS: 64815-80-1) is a synthetic peptide derivative and a chromogenic substrate for enzymes. With a molecular formula of C26H34N8O6 · HCl and a molecular weight of 591.06 g/mol, this compound is primarily utilised in biochemical research for the assay and characterisation of proteolytic enzymes, particularly those involved in coagulation pathways and fibrinolysis. It serves as a valuable reagent in enzyme kinetics and molecular biology studies.

Enzyme Activity Assays
This compound functions as a chromogenic substrate for measuring the activity of specific proteases. Upon enzymatic cleavage, it releases p-nitroanilide, which can be quantified spectrophotometrically, allowing for enzyme kinetics studies.
Biochemical Research
Utilised in academic and industrial research laboratories for investigating enzyme mechanisms, identifying enzyme inhibitors, and studying protein interactions within biological systems.
Coagulation and Fibrinolysis Research
Particularly relevant for studying enzymes involved in the blood coagulation cascade and fibrinolytic system, such as thrombin and plasmin.
Cell Biology Studies
Can be applied in cell biology research to understand cellular processes involving protease activity and signalling pathways.
| Molecular weight | 591.06 |
|---|---|
| Empirical formula | C26H34N8O6 · HCl |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is N-Benzoyl-Val-Gly-Arg p-nitroanilide hydrochloride used for?
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N-Benzoyl-Val-Gly-Arg p-nitroanilide hydrochloride (CAS: 64815-80-1) is primarily used as a chromogenic substrate for the assay and characterisation of proteolytic enzymes, especially those involved in coagulation and fibrinolysis research.
What are the CAS number and molecular formula?
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The CAS number is 64815-80-1, and the molecular formula is C26H34N8O6 · HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product for research purposes. The specific grade and purity are available upon request or on the Certificate of Analysis. We do not represent this product as USP, BP, or EP grade.
How should N-Benzoyl-Val-Gly-Arg p-nitroanilide hydrochloride be handled?
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Handle with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Store at -20°C. The product has a WGK Germany rating of 3, indicating it is hazardous to water.
How is this chemical shipped and exported?
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Tech Serve Solutions is a global supplier and exporter of fine chemicals, adhering to all relevant shipping and export regulations for hazardous materials. Please contact us for specific international shipping details.
How can I request a sample or quote?
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To request a sample or quote for N-Benzoyl-Val-Gly-Arg p-nitroanilide hydrochloride, please visit our website or contact our sales department directly.
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