N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride
N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride (CAS: 54799-93-8) is a synthetic peptide derivative with the molecular formula C33H40N8O6 · HCl. This compound serves as a chromogenic substrate for various proteases, particularly those involved in the coagulation cascade. Its primary application lies in biochemical research for assaying enzyme activity, aiding in the study of cellular processes, enzyme kinetics, and the development of enzyme inhibitors.
- IUPAC
- N-Benzoyl-L-phenylalanyl-L-valyl-L-arginine-4-nitroanilide hydrochloride
Protease Activity Assays
Utilised as a chromogenic substrate to quantify the activity of specific proteases, including thrombin and related enzymes. The release of the p-nitroanilide moiety upon enzymatic cleavage provides a measurable spectrophotometric signal.
Biochemical Research
A key reagent in cell biology and biochemistry for investigating enzyme mechanisms and pathways. It is instrumental in studying proteinase function and interactions within biological systems.
Enzyme Inhibitor Screening
Applied in the screening and characterisation of enzyme inhibitors. By monitoring the substrate's cleavage rate, researchers can assess the efficacy of potential therapeutic agents.
Coagulation Studies
Essential for research into the blood coagulation system, allowing for the study of thrombin generation and activity, which is critical for understanding haemostasis and thrombosis.
| Molecular weight | 681.18 |
|---|---|
| Empirical formula | C33H40N8O6 · HCl |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride used for?
+
This compound is primarily used as a chromogenic substrate for the quantitative assay of protease activity, especially thrombin and other enzymes involved in coagulation. It aids in biochemical research, enzyme kinetics studies, and screening for enzyme inhibitors.
What are the CAS number and formula for N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride?
+
The CAS number is 54799-93-8 and the molecular formula is C33H40N8O6 · HCl.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies this product for research purposes. The specific grade and purity are detailed in the product's specifications. TSS does not represent this product as USP, BP, EP, or any pharmacopoeia grade unless explicitly stated.
What are the safety and handling recommendations for this product?
+
Handling requires appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. The WGK Germany classification is 3, indicating a hazard to water. Always consult the Safety Data Sheet (SDS) before use.
How is this chemical shipped and exported?
+
Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals. Products are packaged and shipped in compliance with international regulations for chemical transport.
How can I request a sample or quote?
+
To request a sample or a quote for N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride, please contact our sales department through the website or by calling our direct line.
▶ Related products
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (C8H13N3O4) — chemical structure, CAS 138472-01-2; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F138472-01-2.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
C8H13N3O4
Biochemicals & Reagents![(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide (C14H18N4O4) — chemical structure, CAS 162626-99-5; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F162626-99-5.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide
C14H18N4O4
Biochemicals & Reagents
(±)-2,5-Dimethoxy-4-bromoamphetamine hydrobromide
Biochemicals & Reagents
(±)-2-(p-Methoxyphenoxy)propionic acid
C10H12O4
Biochemicals & Reagents
(±)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide
C10H13NO2·HBr
Biochemicals & Reagents
(±)-2-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxyoctanoic acid
C8H16O3
Biochemicals & Reagents▶ Explore more
3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis
3-Methyl-1,5-pentanediol
Chemical Synthesis
3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis