N-Benzoyl-Pro-Phe-Arg-p-nitroanilide hydrochloride
N-Benzoyl-Pro-Phe-Arg-p-nitroanilide hydrochloride (CAS: 59188-28-2) is a synthetic peptide substrate with the molecular formula C33H38N8O6 · HCl and a molecular weight of 679.17. This compound is primarily utilised as a biochemical reagent for the study of enzyme activity, specifically in the context of proteases and kallikreins. It serves as a valuable tool in biochemical research, cell biology, and related life science applications.

Enzyme Substrate Studies
This compound functions as a chromogenic substrate for specific proteases, enabling the spectrophotometric detection and quantification of enzyme activity. Its unique structure allows for the release of a coloured product upon cleavage by target enzymes, facilitating kinetic studies.
Biochemical Research Reagent
Utilised in research laboratories to investigate enzyme kinetics, inhibitor screening, and the characterisation of protease function. It is particularly relevant in studies involving kallikrein family enzymes.
Cell Biology Research
Serves as a tool in cell biology to explore cellular processes involving proteases, such as signal transduction pathways and protein turnover. Its application helps in understanding complex biological mechanisms at the cellular level.
| Molecular weight | 679.17 |
|---|---|
| Empirical formula | C33H38N8O6 · HCl |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is N-Benzoyl-Pro-Phe-Arg-p-nitroanilide hydrochloride used for?
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N-Benzoyl-Pro-Phe-Arg-p-nitroanilide hydrochloride is primarily used as a chromogenic substrate for the study of protease and kallikrein enzyme activity in biochemical and cell biology research.
What are the CAS number and formula for this compound?
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The CAS number is 59188-28-2 and the molecular formula is C33H38N8O6 · HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product as a research-grade reagent. We do not represent it as USP, BP, EP, or any pharmacopoeia grade unless explicitly stated. Please refer to the product's Certificate of Analysis for specific purity details.
How should N-Benzoyl-Pro-Phe-Arg-p-nitroanilide hydrochloride be handled and stored?
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Handle with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Store at -20°C to maintain stability. This compound has a WGK Germany rating of 3, indicating it is hazardous.
Is this product controlled or scheduled?
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Based on the provided data, there is no indication that N-Benzoyl-Pro-Phe-Arg-p-nitroanilide hydrochloride is a controlled or scheduled substance. However, users should always verify current local regulations.
How is this chemical packed, shipped, and exported?
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Tech Serve Solutions packs and ships chemicals in compliance with all relevant international and domestic regulations for hazardous materials. We are experienced global exporters of fine chemicals.
How can I request a sample or quote?
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To request a sample or a quote, please contact our sales department directly via the contact information provided on our website, or use the product inquiry form for N-Benzoyl-Pro-Phe-Arg-p-nitroanilide hydrochloride.
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