Nα-Benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide
Nα-Benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide (CAS 59068-47-2) is a synthetic chromogenic peptide substrate with the molecular formula C32H43N9O9. This compound, with a molecular weight of 697.74 g/mol, is primarily utilised in biochemical research for the assay of enzyme activity. It is a valuable tool for scientists studying protease function and kinetics, offering a reliable method for quantitative analysis.

Enzyme Activity Assays
This substrate is designed for the quantitative determination of enzyme activity, particularly for proteases that cleave peptide bonds. The release of the chromophore (4-nitroanilide) upon enzymatic hydrolysis allows for spectrophotometric monitoring.
Protease Research
It serves as a critical reagent in studies investigating the kinetics and mechanisms of proteases. Researchers use it to understand enzyme specificity, inhibition, and activation in various biological contexts.
Biochemical Research Tool
As a fine chemical supplied by Tech Serve Solutions, it is employed in general biochemical laboratories for research purposes. Its defined structure and reactivity make it suitable for developing and validating enzyme assays.
| Molecular weight | 697.74 |
|---|---|
| Empirical formula | C32H43N9O9 |
| Assay | ≥97.0% (HPLC) |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Nα-Benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide used for?
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Nα-Benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide is primarily used as a chromogenic substrate for the quantitative assay of enzyme activity, especially in the study of proteases and their kinetics.
What is the CAS number and molecular formula for this compound?
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The CAS number is 59068-47-2, and the molecular formula is C32H43N9O9.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with a purity of ≥97.0% by HPLC. This is a research grade material, and TSS does not represent it as meeting USP, BP, or EP pharmacopoeia standards.
How should Nα-Benzoyl-L-isoleucyl-L-glutamyl-glycyl-L-arginine-4-nitroanilide be handled and stored?
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This compound requires careful handling using appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or P1 (EN143) respirator filter. It should be stored at -20°C to maintain stability.
Is this compound considered hazardous?
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Yes, according to the WGK Germany classification, it has a hazard rating of 3, indicating significant potential hazards. Always refer to the Safety Data Sheet (SDS) for detailed hazard information and handling procedures.
How is this chemical packed and exported?
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Tech Serve Solutions professionally packs and exports fine chemicals globally, ensuring compliance with all relevant shipping regulations for research reagents.
How can I request a quote or sample?
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To request a quote or sample, please contact the TSS sales department through our website or by phone, providing the product name and CAS number (59068-47-2).
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