Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride
Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride (CAS 21653-40-7) is a synthetic compound with the molecular formula C19H22N6O4 · HCl and a molecular weight of 434.88 g/mol. It serves as a chromogenic substrate, primarily employed in biochemical research for the study of enzyme activity, particularly trypsin. This reagent is essential for quantitative analysis of protease kinetics and characterisation of enzyme mechanisms in cell biology and related fields.
- IUPAC
- L-BAPA,L-BAPNA,BANI
Enzyme Substrate for Protease Assays
Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride is a highly specific chromogenic substrate for trypsin and other serine proteases. Its hydrolysis by the enzyme releases 4-nitroanilide, which can be quantified spectrophotometrically, allowing for the determination of enzyme activity and kinetics.
Biochemical Research Tool
This compound is widely used in molecular biology and biochemistry laboratories to investigate enzyme mechanisms, screen for enzyme inhibitors, and study cellular processes involving proteolysis. Its reliable performance makes it a standard reagent in academic and industrial research settings.
Cell Biology Studies
In cell biology, Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride can be utilised to assess protease activity within cellular environments or in purified enzyme preparations involved in cell signalling and regulation pathways.
| Molecular weight | 434.88 |
|---|---|
| Empirical formula | C19H22N6O4 · HCl |
| Assay | ≥99% (TLC) |
| Storage temperature | −20°C |
| Suitability | suitable for substrate for trypsin |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride used for?
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It is primarily used as a chromogenic substrate for trypsin and other serine proteases in biochemical assays to study enzyme activity and kinetics.
What is the CAS number and molecular formula for Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride?
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The CAS number is 21653-40-7, and the molecular formula is C19H22N6O4 · HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride with an assay of ≥99% (TLC). This product is suitable for research purposes and is not represented as USP, BP, EP, or pharmacopoeia grade.
What are the safety and handling precautions for this chemical?
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Handle with appropriate personal protective equipment including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. The product is classified under WGK Germany 3, indicating it is hazardous to water.
How is Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride shipped and exported?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, providing reliable shipping and export services for Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride worldwide.
How can I request a sample or quote for Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride?
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To request a sample or obtain a quote, please contact our sales team through the website or by phone, providing the product name and CAS number (21653-40-7).
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