Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride
Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride (CAS: 911-77-3) is a key biochemical reagent with the molecular formula C19H22N6O4 · HCl. This compound serves as a specific chromogenic substrate for amidase enzymes, particularly trypsin. It is widely utilised in biochemical research for enzyme activity assays and for studying proteases. Its reliable performance makes it a valuable tool in molecular biology and cell signalling investigations.
- IUPAC
- Nα-Benzoyl-DL-arginine p-nitroanilide hydrochloride,BANI,BAPNA
- Synonyms
- Nα-Benzoyl-DL-arginine p-nitroanilide hydrochloride

Enzyme Substrate
Utilised as a chromogenic substrate for the quantitative assay of amidase enzyme activity, specifically for enzymes like trypsin. It allows for spectrophotometric monitoring of enzyme kinetics.
Biochemical Research
Essential for research in biochemistry and molecular biology, particularly in studies involving protease activity and enzyme mechanisms. Facilitates the investigation of cellular processes.
Cell Biology Studies
Applicable in cell biology for investigating signalling pathways and cellular functions regulated by proteases. Aids in understanding enzyme-substrate interactions within cellular environments.
| Molecular weight | 434.88 |
|---|---|
| Empirical formula | C19H22N6O4 · HCl |
| Assay | ≥98% |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride used for?
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Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride (CAS: 911-77-3) is primarily used as a chromogenic substrate for the assay of amidase enzymes, particularly trypsin, in biochemical and cell biology research.
What are the CAS number and chemical formula for this compound?
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The CAS number is 911-77-3 and the chemical formula is C19H22N6O4 · HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride with an assay of ≥98%. This product is intended for research use and is not represented as USP, BP, EP, or pharmacopoeia grade.
What are the safety and handling considerations?
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This compound requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. It is classified under WGK Germany 3, indicating a high hazard level.
How is this chemical packed and shipped?
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TSS exports fine chemicals globally. Packaging and shipping are handled according to international regulations to ensure product integrity and safety during transit. Specific details are available upon request.
How can I request a quote or sample?
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To request a quote or sample of Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, please contact our sales team via the website or direct inquiry, providing your specific requirements.
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