Nα-Benzoyl-L-arginine-7-amido-4-methylcoumarin hydrochloride
Nα-Benzoyl-L-arginine-7-amido-4-methylcoumarin hydrochloride (CAS: 83701-04-6) is a fluorogenic peptide substrate with the molecular formula C23H25N5O4 · HCl and a molecular weight of 471.94 g/mol. This compound is primarily utilised in biochemical research as a specific substrate for enzymes like trypsin and papain. It plays a crucial role in the study of enzyme kinetics, cell signalling pathways, and protease activity.

Enzyme Substrate for Protease Assays
Utilised as a fluorogenic substrate for the quantitative assay of proteases, particularly trypsin and papain. Cleavage by the enzyme releases a fluorescent 7-amino-4-methylcoumarin (AMC) moiety, enabling sensitive detection and measurement of enzyme activity.
Biochemical Research Tool
Serves as a valuable reagent in cell biology and biochemistry research. It aids in the investigation of enzyme mechanisms, the screening of enzyme inhibitors, and the characterisation of protease function in various biological systems.
Cell Signalling and Neuroscience Studies
Applied in research focusing on cell signalling pathways and neuroscience, where proteases play critical roles. Its use helps elucidate complex biological processes involving enzymatic cleavage of peptide bonds.
| Molecular weight | 471.94 |
|---|---|
| Empirical formula | C23H25N5O4 · HCl |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Nα-Benzoyl-L-arginine-7-amido-4-methylcoumarin hydrochloride used for?
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This compound is used as a fluorogenic substrate in biochemical research for assaying the activity of proteases such as trypsin and papain, and for studying enzyme kinetics and cell signalling pathways.
What are the CAS number and molecular formula?
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The CAS number is 83701-04-6 and the molecular formula is C23H25N5O4 · HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product for research purposes. The specific assay and purity are detailed in the product specifications. We do not represent this product as USP, BP, EP, or for pharmaceutical use.
How should this chemical be handled and stored?
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Handle with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 or P1 respirator filter. Store at -20°C as indicated.
Does Tech Serve Solutions export this product?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals, including Nα-Benzoyl-L-arginine-7-amido-4-methylcoumarin hydrochloride.
How can I request a quote or sample?
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Please contact our sales team through the website or by phone to request a quote or a sample of Nα-Benzoyl-L-arginine-7-amido-4-methylcoumarin hydrochloride.
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