L-Isoleucine
L-Isoleucine (CAS: 73-32-5) is an essential alpha-amino acid with the chemical formula C2H5CH(CH3)CH(NH2)CO2H and a molecular weight of 131.17 g/mol. This white crystalline powder, derived from non-animal sources, plays a crucial role in protein synthesis and metabolic processes. It is widely used in biochemical research, cell culture applications, and as a component in nutritional supplements, meeting stringent testing specifications for various applications.
- IUPAC
- (2S,3S)-2-Amino-3-methylpentanoic acid

Biochemical Research
L-Isoleucine serves as a fundamental building block in biochemical studies, protein analysis, and metabolic pathway investigations. Its specific stereochemistry and functional groups make it valuable for in vitro research and experimental designs.
Cell Culture Media
This amino acid is a vital component in cell culture media, supporting cell growth, proliferation, and protein synthesis in various laboratory settings. Its suitability for cell culture is confirmed by specific testing.
Nutritional Science
As an essential amino acid, L-Isoleucine is incorporated into nutritional formulations and supplements. It plays a role in muscle metabolism and energy regulation, contributing to overall health and wellness.
Analytical Standards
With a high assay of 98.5-101.0%, L-Isoleucine can be employed as an analytical standard or reference material in chromatographic and spectroscopic analyses, ensuring accuracy in scientific measurements.
| Molecular weight | 131.17 |
|---|---|
| Linear formula | C2H5CH(CH3)CH(NH2)CO2H |
| Assay | 98.5-101.0% |
| Form | powder |
| Solubility | 1 M HCl: soluble50 mg/mL |
| Impurities tested | endotoxin, tested |
| Suitability | meets EP, JP, USP testing specificationssuitable for cell culture |
| Melting point | 288 °C (dec.)(lit.) |
| Biological source | from non-animal source |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 1 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is L-Isoleucine used for?
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L-Isoleucine is used in biochemical research, cell culture media, nutritional science, and as an analytical standard. It is essential for protein synthesis and metabolic processes.
What are the CAS number and chemical formula for L-Isoleucine?
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The CAS number for L-Isoleucine is 73-32-5, and its chemical formula is C2H5CH(CH3)CH(NH2)CO2H.
What grade and purity does Tech Serve Solutions supply for L-Isoleucine?
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Tech Serve Solutions supplies L-Isoleucine with an assay of 98.5-101.0%. It meets EP, JP, and USP testing specifications and is suitable for cell culture. TSS does not represent this product as USP, BP, or EP grade unless explicitly stated.
What are the safety and handling recommendations for L-Isoleucine?
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L-Isoleucine has a WGK Germany rating of 1. When handling, appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter, should be used. It decomposes at 288 °C.
How is L-Isoleucine packed, shipped, and exported?
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L-Isoleucine is supplied as a powder and is carefully packed for secure transit. Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, facilitating international shipments.
How can I request a sample or quote for L-Isoleucine?
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To request a sample or a quote for L-Isoleucine, please contact our sales department through the website or by phone. Our team will be happy to assist you with your specific requirements.
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