Iodonitrotetrazolium chloride
Iodonitrotetrazolium chloride (CAS: 146-68-9) is a chemical compound with the empirical formula C19H13ClIN5O2 and a molecular weight of 505.70 g/mol. This tetrazole derivative is primarily utilised as an electron acceptor in the colorimetric assay of various dehydrogenases. Its properties make it a valuable reagent in biochemical research and diagnostic applications where enzymatic activity needs to be quantified.
- IUPAC
- 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride,p-Iodonitrotetrazolium Violet,INT
- Synonyms
- 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloridep-Iodonitrotetrazolium VioletINT

Enzyme Activity Assays
Iodonitrotetrazolium chloride serves as an effective electron acceptor for the colorimetric determination of dehydrogenase activity. It enables sensitive and quantitative measurement of enzyme function in biological samples.
Biochemical Research
This compound is a key reagent in various biochemical studies, facilitating the investigation of metabolic pathways and enzyme kinetics. Its use aids in understanding cellular processes and disease mechanisms.
Diagnostic Kits
The colorimetric properties of Iodonitrotetrazolium chloride make it suitable for incorporation into diagnostic kits designed to assess specific enzymatic activities relevant to health conditions.
| Molecular weight | 505.70 |
|---|---|
| Empirical formula | C19H13ClIN5O2 |
| Assay | 95% |
| Solubility | H2O: soluble4 mg/mL |
| Storage temperature | 2-8°C |
| Application | Electron acceptor for the colorimetric assay of various dehydrogenases |
| Melting point | 240 °C (dec.)(lit.) |

Hazard statements
- H371May cause damage to organs
Precautionary statements
- P260Do not breathe dust, fume, gas, mist, vapours or spray
| Water hazard class (WGK, DE) | 3 |
|---|---|
| Hazard codes (EU) | Xn |
| Risk statements (R) | 20/21/22-68/20/21/22 |
| Safety statements (S) | 36/37 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Iodonitrotetrazolium chloride used for?
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Iodonitrotetrazolium chloride is primarily used as an electron acceptor in the colorimetric assay of various dehydrogenases.
What is the CAS number and formula for Iodonitrotetrazolium chloride?
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The CAS number is 146-68-9, and the empirical formula is C19H13ClIN5O2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Iodonitrotetrazolium chloride with an assay of 95%. TSS does not represent this product as USP, BP, EP or other pharmacopoeia grades.
How should I safely handle Iodonitrotetrazolium chloride?
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Iodonitrotetrazolium chloride is classified with the GHS symbol GHS08 and signal word Warning. It carries the hazard statement H371, indicating it may cause damage to organs. Handle with appropriate personal protective equipment in a well-ventilated area, and follow all P260 precautionary statements regarding not breathing dust.
How is Iodonitrotetrazolium chloride stored and shipped?
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This product should be stored at 2-8°C. Tech Serve Solutions exports fine chemicals globally, ensuring appropriate packaging and documentation for safe international transit.
How can I request a quote or sample?
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To request a quote or a sample of Iodonitrotetrazolium chloride, please contact our sales team through the website or by phone.
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