Hematoxylin
Hematoxylin (CAS: 517-28-2), with the empirical formula C16H14O6 · xH2O and a molecular weight of 302.28 (anhydrous basis), is a natural dye renowned for its critical role in histological staining. Typically supplied as a powder, this compound is essential for visualising cellular structures, particularly in laboratory research and diagnostic pathology. Its certified grade ensures reliable and reproducible staining results.
- IUPAC
- Natural Black 1

Histological Staining
Hematoxylin is a foundational stain in histology, used extensively for differential staining of cell nuclei. It is typically employed in conjunction with counterstains to enhance the visualisation of tissue morphology in microscopic examination.
Cytological Preparations
This compound serves as a vital stain for cytological samples, allowing for the clear differentiation of cellular components. Its application aids in the accurate analysis of cells obtained from various bodily sources.
Biological Research
As a key reagent in cell biology, Hematoxylin is integral to numerous research protocols requiring precise cellular imaging. Its consistent quality supports reliable experimental outcomes in academic and industrial research settings.
| Molecular weight | 302.28 (anhydrous basis) |
|---|---|
| Empirical formula | C16H14O6 · xH2O |
| Grade | certified by the Biological Stain Commission |
| Form | powder |
| Solubility | 95% ethanol: soluble1 mg/mL |
| Melting point | 200 °C (dec.)(lit.) |

Hazard statements
- H302Harmful if swallowed
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22-36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 75290 |
|---|---|
| Gene Information | rat ... Adora1(29290), Adora2a(25369) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Hematoxylin used for?
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Hematoxylin is primarily used as a stain in histology and cytology to visualise cell nuclei, aiding in the microscopic examination of tissue samples and cellular preparations.
What are the CAS number and formula for Hematoxylin?
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Hematoxylin has the CAS number 517-28-2 and the empirical formula C16H14O6 · xH2O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Hematoxylin certified by the Biological Stain Commission. Purity is based on an anhydrous weight. This product is not intended for USP, BP, EP, or other pharmacopoeia standards.
How should Hematoxylin be handled safely?
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Hematoxylin is harmful if swallowed, causes skin irritation, causes serious eye irritation, and may cause respiratory irritation. When handling, use a dust mask (type N95 US), wear eyeshields and gloves, and ensure adequate ventilation. Avoid breathing dust. P261 is recommended.
Is Hematoxylin a controlled substance?
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The provided data does not indicate that Hematoxylin is a controlled substance.
How is Hematoxylin packed, shipped, and exported?
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Hematoxylin is supplied as a powder. Specific packaging details and export compliance are managed according to international regulations and customer requirements.
How can I request a sample or quote for Hematoxylin?
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To request a sample or a quote for Hematoxylin, please contact the Tech Serve Solutions sales team through our website or direct inquiry channels.
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