Gly-Phe p-nitroanilide

CAS 21027-72-5C17H18N4O4Biochemicals & Reagents

Gly-Phe p-nitroanilide (CAS: 21027-72-5), with the molecular formula C17H18N4O4 and a molecular weight of 342.35 g/mol, is a key biochemical reagent. It functions as a chromogenic substrate for enzymes, particularly cathepsins, enabling their activity to be measured colorimetrically. This compound is widely employed in biochemical research for studying enzyme kinetics and the processes of apoptosis and cell signalling.

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Gly-Phe p-nitroanilide (C17H18N4O4) — chemical structure, CAS 21027-72-5; Life Science, fine chemical supplied by Tech Serve Solutions

Apoptosis EnzymesApplication IndexBiochemicals and ReagentsCathepsin CCell BiologyCell Signaling EnzymesColorimetricEnzyme SubstratesEnzymesInhibitors

01 /Applications

Enzyme Substrate

Gly-Phe p-nitroanilide serves as a chromogenic substrate for proteases such as Cathepsin C. Enzymatic cleavage releases p-nitroaniline, allowing for spectrophotometric quantification of enzyme activity.

Apoptosis Research

Used in studies related to programmed cell death, this substrate can help researchers investigate the role of specific proteases in apoptotic pathways.

Cell Signalling Studies

Its application extends to understanding cell signalling mechanisms where enzymes that cleave this substrate play a critical role.

Biochemical Assays

Facilitates the development and execution of various biochemical assays, particularly those involving enzyme activity detection and measurement.

02 /Properties

Molecular weight	342.35
Empirical formula	C17H18N4O4
Storage temperature	−20°C

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

21027-72-5

MDL number

MFCD00038182

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is Gly-Phe p-nitroanilide used for?

Gly-Phe p-nitroanilide is primarily used as a chromogenic substrate in biochemical assays to measure the activity of specific enzymes, such as Cathepsin C, and in research related to apoptosis and cell signalling.

What is the CAS number and molecular formula for Gly-Phe p-nitroanilide?

The CAS number is 21027-72-5, and the molecular formula is C17H18N4O4.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies this product for research purposes. The specific grade and purity are available on the product's Certificate of Analysis. TSS does not represent this product as USP, BP, or EP grade.

How should Gly-Phe p-nitroanilide be handled and stored?

Handle with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or P1 (EN143) respirator filter. Store at -20°C. The compound has a WGK Germany classification of 3.

How are your chemicals shipped and exported?

Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, ensuring compliant and secure packaging for international shipments.

How can I request a sample or quote for Gly-Phe p-nitroanilide?

Please contact our customer service team or use the online inquiry form on our website to request a sample or a detailed quote for Gly-Phe p-nitroanilide.