Fluorescent Red Mega 500
Fluorescent Red Mega 500 (CAS 540528-13-0), a highly fluorescent dye with the molecular formula C31H36N2O8S, offers a large Stokes' shift. This characteristic enables its excitation by short wavelength light sources such as argon lasers, making it suitable for multicolor techniques. Its direct conversion of excitation light to emission without FRET mechanisms opens avenues for advanced applications in research, including DNA sequencing and FISH microscopy.
Multicolor Fluorescence Techniques
Fluorescent Red Mega 500 is specifically designed for multicolor applications. Its unique spectral properties allow for independent detection of multiple labels within the same experiment, facilitating complex biological analyses.
FISH Microscopy
The dye's direct conversion of excitation light to emission, coupled with its fluorescence characteristics, makes it well-suited for Fluorescence In Situ Hybridization (FISH) microscopy, enabling precise cellular imaging.
DNA Sequencing
Due to its efficiency and spectral properties, Fluorescent Red Mega 500 has potential applications in DNA sequencing methodologies. Its ability to be excited by standard laser sources is advantageous.
Biochemical Assays
As a fluorescent label and probe, this compound can be utilised in various biochemical assays requiring sensitive detection methods. Its bright red fluorescence is ideal for quantitative measurements.
| Molecular weight | 596.69 |
|---|---|
| Empirical formula | C31H36N2O8S |
| Assay | ≥90% (HPLC) |
| Storage temperature | 2-8°C |
| Application | Fluorescent red Mega 500 and other Mega dyes are specifically designed for multicolor techniques. It is characterized by an extremely large stoke′s shift between excitation and emission maxima. This dye is especially well suited for excitation by argon lasers or other short wavelength excitation light. This means that it can be excited with the same light source or filter arrangement than fluorescein and other widely used dyes as well as other Mega dye. Due to the different emission wavelength several different labels can be detected independently in the same experiment. Due to the small size of the Mega-labels and the direct conversion of excitation light into emission without using a FRET mechanism we foresee a variety of possible applications including DNA sequencing and FISH microscopy. |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 508 nm; λem 608 nm in 0.1 M phosphate pH 7.0 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Fluorescent Red Mega 500 used for?
+
Fluorescent Red Mega 500 is used for multicolor fluorescence techniques, FISH microscopy, DNA sequencing, and other biochemical assays due to its large Stokes' shift and efficient fluorescence.
What is the CAS number and formula for Fluorescent Red Mega 500?
+
The CAS number for Fluorescent Red Mega 500 is 540528-13-0, and its empirical formula is C31H36N2O8S.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies Fluorescent Red Mega 500 with an assay of ≥90% (HPLC). We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
What are the safety and handling precautions for Fluorescent Red Mega 500?
+
When handling Fluorescent Red Mega 500, it is essential to wear eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if respiratory protection is needed. The substance has a WGK Germany classification of 3.
How is Fluorescent Red Mega 500 shipped?
+
Fluorescent Red Mega 500 is typically stored at 2-8°C. Tech Serve Solutions exports fine chemicals globally, adhering to all relevant shipping regulations.
How can I request a quote or sample for Fluorescent Red Mega 500?
+
To request a quote or sample for Fluorescent Red Mega 500, please contact our sales team via the website or your dedicated account manager.
▶ Related products
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (C8H13N3O4) — chemical structure, CAS 138472-01-2; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F138472-01-2.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
C8H13N3O4
Biochemicals & Reagents![(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide (C14H18N4O4) — chemical structure, CAS 162626-99-5; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F162626-99-5.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide
C14H18N4O4
Biochemicals & Reagents
(±)-2,5-Dimethoxy-4-bromoamphetamine hydrobromide
Biochemicals & Reagents
(±)-2-(p-Methoxyphenoxy)propionic acid
C10H12O4
Biochemicals & Reagents
(±)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide
C10H13NO2·HBr
Biochemicals & Reagents
(±)-2-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxyoctanoic acid
C8H16O3
Biochemicals & Reagents
