Fluorescent Red Mega 520

CAS 540528-09-4C26H30N2O7SBiochemicals & Reagents

Fluorescent Red Mega 520 (CAS: 540528-09-4, C26H30N2O7S) is a high-purity fluorescent dye supplied by Tech Serve Solutions. Its large Stokes shift makes it suitable for multicolor techniques, particularly when excited by argon lasers or similar short-wavelength light sources. This dye is designed for direct conversion of excitation light into emission without FRET, enabling applications such as DNA sequencing and FISH microscopy. It is available with an assay of ≥97.0% (HPLC).

IUPAC: 6-[4-(7-Diethylamino-2-oxo-1-benzopyran-3-ylvinyl)-3-sulfonatopyridinio]hexanoic acid

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Fluorescent Red Mega 520 (C26H30N2O7S) — chemical structure, CAS 540528-09-4; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsFluorescent LabelsFluorescent ProbesLabelsParticles and StainsMega Labels

01 /Applications

Multicolor Fluorescence Techniques

Fluorescent Red Mega 520 is designed for multicolor applications, allowing independent detection of multiple labels in the same experiment due to its distinct emission wavelength. It can be excited using the same light sources or filter arrangements as other common fluorescent dyes.

DNA Sequencing

The dye's direct conversion of excitation light to emission and its suitability for short-wavelength excitation make it valuable for applications in DNA sequencing. Its minimal use of FRET mechanisms is advantageous in such precise analytical methods.

FISH Microscopy

Fluorescent Red Mega 520 is well-suited for FISH (Fluorescence In Situ Hybridization) microscopy. Its fluorescent properties and compatibility with standard excitation sources aid in cellular and genetic analysis.

Biochemical Assays

As a fluorescent label and probe, Fluorescent Red Mega 520 can be employed in various biochemical assays where fluorescence detection is required. Its defined excitation and emission spectra are beneficial for quantitative measurements.

02 /Properties

Molecular weight	514.59
Empirical formula	C26H30N2O7S
Assay	≥97.0% (HPLC)
Storage temperature	2-8°C
Application	Fluorescent red Mega 520 and other Mega dyes are specifically designed for multicolor techniques. It is characterized by an extremely large stokes shift between excitation and emission maxima. This dye is especially well suited for excitation by argon lasers or other short wavelength excitation light. This also means that it can be excited with the same light source or filter arrangement as fluorescein and other widely used dyes, as well as other Mega dyes. Due to the different emission wavelengths several different labels can be detected independently in the same experiment. Due to the small size of the Mega labels and the direct conversion of excitation light into emission without using a FRET mechanism, there is a variety of possible applications including DNA sequencing and FISH microscopy.
Suitability	suitable for fluorescence

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

540528-09-4

MDL number

MFCD06798178

PubChem substance

24865840

05 /Additional specifications

Product Line	BioReagent
Fluorescence	λex 527 nm; λem 663 nm in 0.1 M phosphate pH 7.0

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is Fluorescent Red Mega 520 used for?

Fluorescent Red Mega 520 is used for multicolor fluorescence techniques, DNA sequencing, FISH microscopy, and other biochemical assays, owing to its large Stokes shift and efficient fluorescence emission.

What is the CAS number and formula for Fluorescent Red Mega 520?

The CAS number for Fluorescent Red Mega 520 is 540528-09-4, and its empirical formula is C26H30N2O7S.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Fluorescent Red Mega 520 with an assay of ≥97.0% (HPLC). This product is suitable for fluorescence applications and is not represented as USP, BP, EP, or pharmacopoeia grade.

What are the safety and handling recommendations for Fluorescent Red Mega 520?

Handling Fluorescent Red Mega 520 requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. It carries a WGK Germany classification of 3, indicating it is hazardous to water.

How is Fluorescent Red Mega 520 stored and shipped?

Fluorescent Red Mega 520 should be stored at 2-8°C. Tech Serve Solutions exports this chemical globally, adhering to all necessary shipping and handling regulations for fine chemicals.

How can I request a sample or quote for Fluorescent Red Mega 520?

To request a sample or obtain a quote for Fluorescent Red Mega 520, please contact our sales team through the TSS website or by phone. We will promptly assist you with your requirements.