Fluorescent Red Mega 485 NHS-ester

CAS 890317-36-9C29H33N3O9SBiochemicals & Reagents

Fluorescent Red Mega 485 NHS-ester (CAS: 890317-36-9; Formula: C29H33N3O9S; MW: 599.65) is a specialised amine-conjugating fluorescent biolabel. Designed for multicolour techniques, it offers an exceptionally large Stokes' shift, enabling efficient excitation by standard argon lasers and independent detection with other fluorescent dyes. Its direct conversion of excitation light into emission without FRET mechanisms makes it suitable for advanced applications like DNA sequencing and FISH microscopy.

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Fluorescent Red Mega 485 NHS-ester (C29H33N3O9S) — chemical structure, CAS 890317-36-9; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsFluorescent LabelsFluorescent ProbesLabelsParticles and StainsMega Labels

01 /Applications

Amine Conjugation

This NHS-ester derivative is engineered for covalent coupling to proteins and other biomolecules featuring primary amino groups. Its reactive nature allows for stable and specific labelling of biological targets.

Multicolour Fluorescence Assays

The Mega 485 dye's broad excitation spectrum and distinct emission profile make it ideal for multiplexed analyses. It can be used alongside other Mega dyes or traditional fluorophores without spectral overlap.

High-Resolution Microscopy

Due to its efficient fluorescence and minimal spectral overlap, Fluorescent Red Mega 485 NHS-ester is well-suited for techniques such as FISH microscopy, enabling detailed visualisation of cellular structures.

DNA Sequencing

The dye's characteristics, including its excitation by common laser sources and direct fluorescence emission, suggest potential applications in advanced DNA sequencing methodologies.

02 /Properties

Molecular weight	599.65
Empirical formula	C29H33N3O9S
Assay	≥90% (coupling to amines)
Storage temperature	2-8°C
Application	Fluorescent Red Mega 485 NHS-ester is an amine conjugating fluorescent biolabel that can be used for covalent coupling to proteins and other biomolecules containing primary amino groups.Fluorescent red Mega 485 and other Mega dyes are specifically designed for multicolor techniques. It is characterized by an extremely large stoke′s shift between excitation and emission maxima. This dye is especially well suited for excitation by argon lasers or other short wavelength excitation light. This means that it can be excited with the same light source or filter arrangement than fluorescein and other widely used dyes as well as other Mega dye. Due to the different emission wavelength several different labels can be detected independently in the same experiment. Due to the small size of the Mega-labels and the direct conversion of excitation light into emission without using a FRET mechanism we foresee a variety of possible applications including DNA sequencing and FISH microscopy.
Suitability	suitable for fluorescence

03 /Safety & handling

GHS hazard pictogram: Corrosive (GHS05) — Corrosive

Danger

Hazard statements

H318Causes serious eye damage

Precautionary statements

P280Wear protective gloves, clothing and eye/face protection
P305IF IN EYES

Water hazard class (WGK, DE)	3
Hazard codes (EU)	Xi
Risk statements (R)	41
Safety statements (S)	26-39

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

890317-36-9

MDL number

MFCD06798200

05 /Additional specifications

Product Line	BioReagent
Fluorescence	λex 482 nm; λem 559 nm in 0.1 M phosphate pH 7.0

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is Fluorescent Red Mega 485 NHS-ester used for?

Fluorescent Red Mega 485 NHS-ester is used as an amine-conjugating fluorescent biolabel for covalent coupling to proteins and biomolecules with primary amino groups. It is particularly designed for multicolour techniques, DNA sequencing, and FISH microscopy due to its large Stokes' shift and efficient excitation.

What is the CAS number and molecular formula for Fluorescent Red Mega 485 NHS-ester?

The CAS number is 890317-36-9, and the empirical formula is C29H33N3O9S.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies this product with an assay of ≥90% (coupling to amines), suitable for fluorescence applications. It is provided as a research-grade chemical and is not represented as USP, BP, or EP grade.

How should Fluorescent Red Mega 485 NHS-ester be handled and stored?

This compound causes serious eye damage (H318) and requires specific handling precautions. Wear eye protection (P280-P305 + P351 + P338). Store at 2-8°C. It is classified as GHS05 'Danger' and has a WGK Germany rating of 3.

Is Fluorescent Red Mega 485 NHS-ester available for export?

Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including Fluorescent Red Mega 485 NHS-ester, and can fulfil international orders.

How can I request a sample or quote?

To request a sample or a quotation, please contact our sales department through the website or by phone, providing the product name and CAS number (890317-36-9).