Ethylenediaminetetraacetic acid
Ethylenediaminetetraacetic acid (EDTA), CAS 60-00-4, with the linear formula (HO2CCH2)2NCH2CH2N(CH2CO2H)2 and a molecular weight of 292.24 g/mol, is a versatile chelating agent. It is widely employed in analytical chemistry for complexometric titrations and in industrial processes to sequester metal ions, preventing their detrimental effects. EDTA is also crucial in biochemical research and certain formulation applications.
- IUPAC
- (Ethylenedinitrilo)tetraacetic acid,EDTA,Edathamil,Ethylenedinitrilotetraacetic acid
- Synonyms
- EDTAEdathamil(Ethylenedinitrilo)tetraacetic acidEthylenedinitrilotetraacetic acid

Analytical Chemistry Reagent
EDTA is extensively used as a complexometric titrant for the quantitative determination of metal ions. Its ability to form stable, water-soluble complexes with a wide range of metal cations makes it invaluable in this field.
Industrial Scale Metal Ion Sequestration
In various industrial applications, EDTA effectively sequesters problematic metal ions. This prevents metal-induced precipitation, decomposition, or interference in processes such as water treatment, pulp and paper manufacturing, and textile processing.
Biochemical and Cell Culture Applications
Suitable for cell culture, EDTA is used to chelate divalent metal cations like calcium and magnesium, which can be crucial for maintaining cell viability and preventing cell clumping. It is also used in laboratory reagents for biological research.
Component in Detergents and Cleaning Agents
EDTA enhances the effectiveness of detergents and cleaning agents by complexing with calcium and magnesium ions present in hard water, thereby preventing soap scum formation and improving overall cleaning performance.
| Molecular weight | 292.24 |
|---|---|
| Linear formula | (HO2CCH2)2NCH2CH2N(CH2CO2H)2 |
| Assay | 98.0% |
| Refractive index | n20/D 1.363 |
| pH | 8.0±0.2 |
| Concentration | 0.02% in DPBS (0.5 mM) |
| Storage temperature | 2-8°C |
| Melting point | 250 °C (dec.)(lit.) |
| Impurities tested | endotoxin, tested |
| Suitability | suitable for cell culture |

Hazard statements
- H319Causes serious eye irritation
Precautionary statements
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Transport (UN / ADR) | UN 3077 9 / PGIII |
| Water hazard class (WGK, DE) | 2 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36 |
| Safety statements (S) | 26 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Uv Absorption | λ: 280 nm Amax: ≤0.25 |
|---|---|
| Absorption | absorption/268 nm cut-off |
| Sterility | sterile-filtered |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Ethylenediaminetetraacetic acid used for?
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Ethylenediaminetetraacetic acid (EDTA) is primarily used as a chelating agent to sequester metal ions. Its applications span analytical chemistry, industrial processes, biochemical research, cell culture, and as a component in cleaning formulations.
What are the CAS number and formula for Ethylenediaminetetraacetic acid?
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The CAS number for Ethylenediaminetetraacetic acid is 60-00-4, and its linear formula is (HO2CCH2)2NCH2CH2N(CH2CO2H)2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Ethylenediaminetetraacetic acid with an assay of 98.0%. We do not represent this product as USP, BP, or EP grade unless explicitly stated in the product documentation.
How should Ethylenediaminetetraacetic acid be handled safely?
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Handle with care, wearing a dust mask type N95 (US), eyeshields, and gloves. It is classified as causing serious eye irritation (H319). In case of contact with eyes, rinse cautiously with water for several minutes, removing contact lenses if present and easy to do. Continue rinsing. Store at 2-8°C.
How is Ethylenediaminetetraacetic acid packed, shipped, and exported?
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Ethylenediaminetetraacetic acid is packed according to industry standards for safe transport and is available for global export. Specific shipping and packing details can be provided upon request.
How can I request a sample or quote for Ethylenediaminetetraacetic acid?
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To request a sample or a quote for Ethylenediaminetetraacetic acid, please contact our sales team through the website or by phone, providing your specific requirements and shipping details.
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