Biotin disulfide N-hydroxysuccinimide ester
Biotin disulfide N-hydroxysuccinimide ester (CAS: 142439-92-7; Formula: C22H33N5O7S3; MW: 575.72) is a biotinylation reagent featuring a cleavable disulfide linkage and a spacer arm. It is primarily used for the modification of primary amines in biological samples, facilitating protein structural analysis and proteomic studies. This compound is supplied as a powder for research applications.
- IUPAC
- (2-[Biotinamido]ethylamido)-3,3′-dithiodipropionic acid N-hydroxysuccinimide ester
Amine Biotinylation
This reagent enables the covalent attachment of biotin to primary amine groups, commonly found on proteins and other biomolecules. The reaction is typically performed in the pH range of 6.5-8.5.
Disulfide Bond Cleavage
The disulfide bond within the spacer arm can be cleaved under reducing conditions, allowing for mild recovery of the biotinylated compound or further manipulation of the biotinylated molecule.
Protein Structural Analysis
Biotinylation using this ester can be employed as a tool in protein structural studies, aiding in the characterization and mapping of protein interactions and modifications.
Proteomics Research
In proteomics, this reagent is valuable for labeling proteins and peptides, facilitating their detection, purification, and analysis in complex biological samples.
| Molecular weight | 575.72 |
|---|---|
| Empirical formula | C22H33N5O7S3 |
| Assay | ≥90% (TLC) |
| Form | powder |
| Solubility | DMF: soluble50 mg/mL |
| Storage temperature | −20°C |
| Application | Biotinylation reagent incorporating a spacer with a cleavable disulfide linkage. Typically coupled to primary amine in the pH range 6.5-8.5. Cleavage of disulfide requires reducing agent allowing mild recovery of biotinylated compound. |
Hazard statements
- H360D
Precautionary statements
- P201Obtain special instructions before use
- P308IF exposed or concerned
| Water hazard class (WGK, DE) | 3 |
|---|---|
| Hazard codes (EU) | T |
| Risk statements (R) | 61 |
| Safety statements (S) | 53-45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Biotin disulfide N-hydroxysuccinimide ester used for?
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Biotin disulfide N-hydroxysuccinimide ester is used as a biotinylation reagent for modifying primary amines in biomolecules. It incorporates a cleavable disulfide linkage, useful for protein structural analysis, proteomic studies, and molecular biology applications.
What are the CAS number and formula for Biotin disulfide N-hydroxysuccinimide ester?
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The CAS number is 142439-92-7, and the empirical formula (Hill notation) is C22H33N5O7S3.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Biotin disulfide N-hydroxysuccinimide ester with an assay of ≥90% (TLC). This is a research-grade chemical and is not represented as USP, BP, or EP grade.
How should Biotin disulfide N-hydroxysuccinimide ester be handled and stored?
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This compound is classified as Danger, with hazard statement H360D (May damage the unborn child). It requires careful handling to avoid exposure. Store at -20°C.
Is Biotin disulfide N-hydroxysuccinimide ester packed, shipped, or exported by TSS?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including Biotin disulfide N-hydroxysuccinimide ester, which can be packed and shipped worldwide.
How can I request a sample or quote for Biotin disulfide N-hydroxysuccinimide ester?
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To request a sample or obtain a quote, please contact our customer service team through the website or by calling our sales department.
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