Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt
Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt (CAS: 119616-38-5; Formula: C14H18N3NaO8S2; MW: 443.43) is a water-soluble biotinylation reagent. It is primarily used to covalently label primary amines in biological molecules, particularly proteins, within a pH range of 6.5 to 8.5. This reagent is instrumental in molecular biology, proteomics, and protein structural analysis for applications such as affinity purification and detection.
- IUPAC
- Sulfosuccinimidyl biotin
- Synonyms
- Sulfosuccinimidyl biotin
Biotinylation of Primary Amines
This sulfosuccinimidyl ester derivative of biotin is designed to react with primary amine groups (e.g., lysine residues in proteins) under mild aqueous conditions, typically at pH 6.5-8.5. This allows for the specific labelling of biomolecules for subsequent detection or purification.
Protein Modification and Analysis
Its water solubility makes it ideal for modifying proteins in biological buffers without the need for organic co-solvents. The resulting biotinylated proteins can be utilized in various assays, including Western blotting, ELISA, and affinity chromatography.
Proteomics Research
In proteomics, Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt is employed to tag proteins for identification and characterization, aiding in the study of protein-protein interactions and cellular pathways.
Molecular Biology Reagent
This compound serves as a versatile reagent in molecular biology laboratories for immobilising or detecting nucleic acids, proteins, or other biomolecules through biotin-streptavidin interactions.
| Molecular weight | 443.43 |
|---|---|
| Empirical formula | C14H18N3NaO8S2 |
| Assay | ≥90% (TLC) |
| Form | powder |
| Solubility | H2O: soluble10 mg/mLDMF: soluble50 mg/mL |
| Storage temperature | −20°C |
| Application | Water soluble biotinylation reagent. Typically coupled to primary amine in the pH range 6.5-8.5. |
Hazard statements
- H302Harmful if swallowed
- H312Harmful in contact with skin
- H315Causes skin irritation
- H319Causes serious eye irritation
- H332Harmful if inhaled
- H335May cause respiratory irritation
- H361Suspected of damaging fertility or the unborn child
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
| Protective equipment | Eyeshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 63-20/21/22-36/37/38 |
| Safety statements (S) | 22-26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt used for?
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It is a water-soluble biotinylation reagent used to label primary amines in biomolecules, particularly proteins, for applications in molecular biology, proteomics, and protein analysis.
What is the CAS number and chemical formula?
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The CAS number is 119616-38-5 and the chemical formula is C14H18N3NaO8S2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt with an assay of ≥90% (TLC). TSS does not represent this product as USP, BP, EP, or other pharmacopoeial grades.
How should Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt be handled safely?
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This compound is classified as harmful if swallowed, in contact with skin, or if inhaled, and causes skin, eye, and respiratory irritation. Use appropriate personal protective equipment, including eyeshields, gloves, and a suitable respirator, and handle in a well-ventilated area. Refer to the safety data sheet for detailed information.
How is this chemical packed, shipped, and exported?
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This chemical is typically supplied as a powder and stored at -20°C. TSS exports fine chemicals globally, adhering to all relevant shipping and customs regulations.
How can I request a quote or sample?
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Please contact Tech Serve Solutions directly via our website or sales department to request a quote or a sample of Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt.
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