5-Methylisatin
5-Methylisatin (CAS: 608-05-9), with the molecular formula C9H7NO2 and a molecular weight of 161.16 g/mol, is an indole derivative. It is primarily utilised as a chemical synthesis building block, particularly within heterocyclic chemistry. This compound has been employed as an internal standard for the quantitative analysis of isatin in biological samples using gas chromatography-mass spectrometry. Supplied by Tech Serve Solutions for research and development purposes.
- IUPAC
- 5-Methylindole-2,3-dione,NSC 9398

Analytical Standard
5-Methylisatin serves as an internal standard in analytical chemistry, specifically for the quantification of isatin in biological fluids via capillary gas chromatography-mass spectrometry (GC-MS).
Chemical Synthesis Intermediate
As a heterocyclic building block, 5-Methylisatin is valuable in organic synthesis for constructing more complex molecular architectures, including indole-based compounds.
Research and Development
This compound is utilized in laboratory settings for various research applications, aiding in the exploration of new chemical entities and reaction pathways.
| Molecular weight | 161.16 |
|---|---|
| Empirical formula | C9H7NO2 |
| Assay | 95% |
| Solubility | methanol: soluble25 mg/mL, clear to slightly hazy, orange to red |
| Application | 5-Methylisatin was used as an internal standard during the determination of isatin (indole-2, 3-dione) in body fluids by capillary column gas chromatography-mass spectrometry (GC—MS)1. |
| Melting point | 180 °C (dec.)(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Methylisatin used for?
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5-Methylisatin is primarily used as a chemical synthesis building block and has been employed as an internal standard in analytical methods, such as GC-MS for the determination of isatin in body fluids.
What is the CAS number and chemical formula for 5-Methylisatin?
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The CAS number for 5-Methylisatin is 608-05-9, and its chemical formula is C9H7NO2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 5-Methylisatin with an assay of 95%. TSS does not represent this product as USP, BP, or EP grade; it is supplied for research and laboratory use.
What are the safety considerations for handling 5-Methylisatin?
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Handling 5-Methylisatin requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used. It has a WGK rating of 3 in Germany, indicating a high hazard to water.
How is 5-Methylisatin packed and shipped?
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5-Methylisatin is carefully packed according to industry standards to ensure safe transit and is exported globally by Tech Serve Solutions.
How can I request a sample or quote for 5-Methylisatin?
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To request a sample or a quote for 5-Methylisatin, please contact our sales department through the website or by phone.
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