5-(Bromomethyl)fluorescein
5-(Bromomethyl)fluorescein (CAS: 148942-72-7; Formula: C21H13BrO5) is a fluorescent derivative of fluorescein, widely used as a labelling agent and fluorescent probe. Its bromomethyl group allows for facile conjugation to biomolecules. This compound exhibits characteristic excitation and emission spectra, making it valuable for various biochemical and cellular imaging applications where fluorescence detection is required. Tech Serve Solutions supplies this reagent for research and development purposes.
- IUPAC
- 5-BMF
Fluorescent Labelling
The bromomethyl group on 5-(Bromomethyl)fluorescein enables covalent attachment to nucleophilic sites on various molecules, such as proteins and nucleic acids. This facilitates their detection and tracking in biological systems.
Fluorescent Probes
As a fluorescent probe, it can be employed in microscopy and flow cytometry to visualise cellular structures or processes. Its distinct fluorescence properties (λex 492 nm; λem 515 nm) are suitable for such applications.
Biochemical Assays
This compound can be incorporated into assay development for detecting specific biological targets or reactions that involve fluorescence readout. Its chemical structure lends itself to the creation of sensitive detection systems.
| Empirical formula | C21H13BrO5 |
|---|---|
| Assay | ≥95% (sum of tautomers, GC) |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 492 nm; λem 515 nm in 0.1 M Tris pH 9.0 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-(Bromomethyl)fluorescein used for?
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5-(Bromomethyl)fluorescein is primarily used as a fluorescent labelling agent and probe in biochemical research, particularly for conjugation to biomolecules and for use in fluorescence microscopy and flow cytometry.
What is the CAS number and formula for 5-(Bromomethyl)fluorescein?
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The CAS number is 148942-72-7 and the empirical formula is C21H13BrO5.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 5-(Bromomethyl)fluorescein with an assay of ≥95% (sum of tautomers, GC). This product is sold for research and development use and is not represented as USP, BP, EP, or pharmacopoeia grade.
How should 5-(Bromomethyl)fluorescein be handled?
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When handling 5-(Bromomethyl)fluorescein, appropriate personal protective equipment should be used, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The compound has a WGK Germany rating of 3, indicating it is hazardous to water.
How is 5-(Bromomethyl)fluorescein packed and shipped?
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Tech Serve Solutions ensures appropriate packaging and shipping procedures for fine chemicals, adhering to all relevant regulations for export.
How can I request a quote or sample for 5-(Bromomethyl)fluorescein?
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To request a quote or sample, please contact the Tech Serve Solutions sales team via our website or by phone.
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