5-Azido-2-nitrobenzoic acid N-hydroxysuccinimide ester
5-Azido-2-nitrobenzoic acid N-hydroxysuccinimide ester (CAS: 60117-35-3, Formula: C11H7N5O6) is a photoactive, heterobifunctional cross-linking reagent. Its primary application involves coupling to primary amines via an amide bond, followed by UV-induced nitrene formation for rapid, non-specific secondary bonding. This reagent is essential in chemical biology, molecular biology, and proteomics for protein modification and structural analysis.
- IUPAC
- N-(5-Azido-2-nitrobenzoyloxy)succinimide,N-Succinimidyl 5-azido-2-nitrobenzoate
- Synonyms
- N-(5-Azido-2-nitrobenzoyloxy)succinimideN-Succinimidyl 5-azido-2-nitrobenzoate
Heterobifunctional Cross-linking
This reagent enables the formation of covalent linkages between biomolecules. It first reacts with primary amines to form a stable amide bond, and subsequently, upon UV irradiation, generates a reactive nitrene species for non-specific cross-linking.
Protein Modification and Labeling
Utilised in studies requiring targeted modification of proteins. The photoactivatable nature allows for controlled cross-linking, aiding in the analysis of protein structures and interactions.
Chemical Biology Research
Serves as a valuable tool in chemical biology for investigating cellular processes and molecular mechanisms. Its ability to cross-link diverse molecules is critical for understanding biological systems.
| Molecular weight | 305.20 |
|---|---|
| Empirical formula | C11H7N5O6 |
| Assay | ≥97.0% (HPLC) |
| Form | powder |
| Colour | yellow |
| Solubility | ethyl acetate: soluble25 mg/mLDMF: soluble |
| Storage temperature | 2-8°C |
| Application | Photoactive, heterobifunctional cross-linking reagent. Typically, initial reaction couples via ester to primary amine by amide bond formation in the pH range 6.5-8.5. Second bonding occurs during UV irradiation (250-350 nm) via reactive nitrene. The latter bonding is rapid and non-specific. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 5-Azido-2-nitrobenzoic acid N-hydroxysuccinimide ester used for?
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This compound is used as a photoactive, heterobifunctional cross-linking reagent in chemical biology and molecular biology for protein modification, structural analysis, and studying molecular interactions.
What are the CAS number and formula for this product?
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The CAS number is 60117-35-3, and the molecular formula is C11H7N5O6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with a purity of ≥97.0% (HPLC). We do not represent this product as USP, BP, EP, or pharmaceutical grade.
What are the safety and handling precautions for this chemical?
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This compound is classified under WGK Germany 3. Appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or P1 (EN143) respirator filter, should be used. Refer to the safety data sheet for comprehensive handling information.
How is this product shipped and exported?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, ensuring compliant and secure packaging for international shipment.
How can I request a quote or sample?
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To request a quote or sample, please contact our sales team via the website or provide your details through our online enquiry form.
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