4-Nitro-o-phenylenediamine
4-Nitro-o-phenylenediamine (CAS: 99-56-9), with the linear formula O2NC6H3(NH2)2 and a molecular weight of 153.14 g/mol, is an organic compound utilised in chemical synthesis. This substance is primarily recognised for its role in scientific research, particularly in studies investigating mutagenicity and related biochemical pathways. It serves as a valuable building block for chemists requiring specific nitrogen-containing structures in their synthetic routes.
- IUPAC
- 1,2-Diamino-4-nitrobenzene

Mutagenicity Research
4-Nitro-o-phenylenediamine has been employed in research to investigate its potential as a mutagen. Studies, such as those using Salmonella typhimurium strain TA98, explore its in vitro activation into potent mutagenic forms.
Organic Synthesis Intermediate
As a diamine with a nitro group, this compound functions as a versatile building block in organic synthesis. It is used to introduce specific functionalities into more complex molecules.
Chemical Research Reagent
Researchers utilise 4-Nitro-o-phenylenediamine as a reagent in various laboratory investigations, particularly within the fields of organic chemistry and toxicology.
| Molecular weight | 153.14 |
|---|---|
| Linear formula | O2NC6H3(NH2)2 |
| Assay | 98% |
| Solubility | 1 N HCl: ethanol, 1:1: soluble50 mg/mL |
| Application | 4-Nitro-o-phenylenediamine was used to study the in vitro activation of 4-nitro-o-phenylenediamine into a potent mutagen in Salmonella typhimurium strain TA98 1. |
| Melting point | 199-201 °C(lit.) |

Hazard statements
- H302Harmful if swallowed
- H317May cause an allergic skin reaction
Precautionary statements
- P280Wear protective gloves, clothing and eye/face protection
| Protective equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22-43 |
| Safety statements (S) | 36/37 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Ph Range | 5 to 8 |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Nitro-o-phenylenediamine used for?
+
4-Nitro-o-phenylenediamine is used in chemical synthesis and research, notably in studies concerning mutagenicity and as an organic building block.
What are the CAS number and formula for 4-Nitro-o-phenylenediamine?
+
The CAS number is 99-56-9 and the linear formula is O2NC6H3(NH2)2.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies 4-Nitro-o-phenylenediamine with an assay of 98%. This product is not represented as USP, BP, EP, or pharmacopoeia grade.
How should 4-Nitro-o-phenylenediamine be handled safely?
+
Handle with appropriate personal protective equipment including a dust mask (N95), eyeshields, face shields, and gloves. It is classified with GHS07 symbol and 'Warning' signal word, with hazards H302 (Harmful if swallowed) and H317 (May cause an allergic skin reaction).
How is 4-Nitro-o-phenylenediamine shipped and exported?
+
Tech Serve Solutions is an experienced global supplier and exporter of fine chemicals, pharmaceutical intermediates, and laboratory reagents, ensuring compliant packaging and shipping.
How can I request a sample or quote for 4-Nitro-o-phenylenediamine?
+
Please contact our sales team via the website or direct contact details to request a sample or obtain a quotation for 4-Nitro-o-phenylenediamine.
▶ Related products

1-(2-Fluorobenzyl)piperazine
C11H15FN2
Chemical Synthesis
1-(2-Fluorophenyl)biguanide hydrochloride
Chemical Synthesis

1-(2-Fluorophenyl)cyclopropanecarboxylic acid
C10H9FO2
Chemical Synthesis
1-(2-Fluorophenyl)piperazine
C10H13FN2
Chemical Synthesis
1-(2-Fluorophenyl)piperazine monohydrochloride
C10H13FN2 · HCl
Chemical Synthesis
1-(2-Furoyl)-1H-benzotriazole
C11H7N3O2
Chemical Synthesis
1-(2-Furoyl)piperazine
C9H12N2O2
Chemical Synthesis
1-(2-Hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione
Chemical Synthesis
▶ Explore more

3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis

3-Methyl-1,5-pentanediol
Chemical Synthesis

3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis