4-Methylumbelliferyl β-D-glucopyranoside
4-Methylumbelliferyl β-D-glucopyranoside (CAS: 18997-57-4) is a fluorogenic substrate with the molecular formula C16H18O8 and a molecular weight of 338.31 g/mol. This compound is widely utilised in biochemical research and diagnostics as a sensitive indicator for the activity of β-glucosidase enzymes. Upon enzymatic cleavage, it releases the highly fluorescent 4-methylumbelliferone, enabling precise quantification of enzyme activity in various biological samples.
- IUPAC
- MUD

Enzyme Activity Assays
4-Methylumbelliferyl β-D-glucopyranoside serves as a chromogenic substrate for the detection and quantification of β-glucosidase enzyme activity. This is crucial in biochemical research and diagnostic kits for monitoring enzyme levels.
Biochemical Research
Its ability to produce a fluorescent signal upon hydrolysis makes it an invaluable tool in glycobiology studies, enzyme kinetics, and post-translational modification research.
Fluorescent Probes
The compound acts as a fluorescent probe, emitting light at specific wavelengths after enzymatic reaction, allowing for sensitive detection in various biological matrices.
Molecular Biology Applications
Used in molecular biology for assays involving glycosidases, aiding in the understanding of cellular processes and metabolic pathways.
| Molecular weight | 338.31 |
|---|---|
| Empirical formula | C16H18O8 |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 316 nm; λem 372 nm (pH 9.1)λex 360 nm; λem 449 nm (Reaction product) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Methylumbelliferyl β-D-glucopyranoside used for?
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4-Methylumbelliferyl β-D-glucopyranoside is primarily used as a fluorogenic substrate for the assay of β-glucosidase activity in biochemical research, diagnostics, and molecular biology applications.
What is the CAS number and chemical formula for 4-Methylumbelliferyl β-D-glucopyranoside?
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The CAS number is 18997-57-4, and the empirical formula is C16H18O8.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product for research and laboratory use. The assay is typically determined by the specific batch and available upon request. We do not represent this product as USP, BP, EP, or for human/veterinary use unless explicitly stated.
What are the safety and handling precautions for this chemical?
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Handle with appropriate personal protective equipment including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. Dispose of waste according to local regulations. This compound has a WGK Germany rating of 3.
How is this chemical packed, shipped, and exported?
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This chemical is typically stored at -20°C and packaged in compliance with international shipping regulations for laboratory reagents. TSS is a specialist global supplier and exporter.
How can I request a sample or a quote?
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Please contact our sales team via the website or direct email to request a quotation or a product sample.
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