4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate
4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate (CAS 53643-12-2) is a fluorogenic substrate utilised in biochemical research. With a molecular weight of 582.55 g/mol (anhydrous basis), this compound serves as a valuable tool for detecting and quantifying enzyme activity, particularly chitinases and related glycosidases. Its fluorescent properties allow for sensitive detection in various biological assays.
- IUPAC
- 4-Methylumbelliferyl-N,N′-diacetyl-β-D-chitobioside hydrate
- Synonyms
- 4-Methylumbelliferyl-N,N′-diacetyl-β-D-chitobioside hydrate
Enzyme Activity Assays
This compound functions as a specific fluorogenic substrate for enzymes involved in carbohydrate metabolism. Upon cleavage by the target enzyme, it releases a fluorescent product, enabling sensitive detection and quantification of enzyme activity in biological samples.
Glycobiology Research
As a chitobioside derivative, it is instrumental in studying the structure and function of complex carbohydrates and glycosylation pathways. Researchers utilise it to investigate enzyme mechanisms and substrate specificities within glycobiology.
Molecular Biology Tools
It serves as a reagent in molecular biology for assays investigating enzyme kinetics and inhibition. The fluorescent readout provides a quantitative measure for screening potential enzyme modulators or studying enzymatic processes.
| Molecular weight | 582.55 (anhydrous basis) |
|---|---|
| Empirical formula | C26H34N2O13 · xH2O |
| Assay | ≥98% (TLC) |
| Solubility | DMF: soluble10 mg/mL, clear, colorless |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 330 nm; λem 375 nm in methanol |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate used for?
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4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate is primarily used as a fluorogenic substrate in biochemical and molecular biology research for the detection and quantification of enzyme activity, particularly chitinases and related glycosidases.
What is the CAS number and formula for this compound?
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The CAS number is 53643-12-2. The empirical formula is C26H34N2O13 · xH2O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with an assay of ≥98% (TLC). This product is intended for research and laboratory use and is not represented as USP, BP, EP, or other pharmacopoeial grades.
How should 4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate be handled?
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Handle with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Ensure adequate ventilation and avoid inhalation or contact with skin and eyes. Refer to the Safety Data Sheet for detailed handling instructions.
How is this chemical shipped and exported?
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Tech Serve Solutions specialises in the global supply and export of fine chemicals. This product is typically shipped under conditions suitable for maintaining its stability, such as at −20°C, adhering to all relevant shipping regulations.
How can I request a quote or sample?
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To request a quote or a sample, please use the contact form on our website or contact our sales department directly. Our team will be pleased to assist you with your specific requirements.
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