2-Iminothiolane hydrochloride
2-Iminothiolane hydrochloride (CAS 4781-83-3, C4H7NS · HCl, MW 137.63) is a thiolating reagent crucial for protein modification and structural analysis. It reacts with primary amines under mild alkaline conditions (pH 7-10) via an amidine bond, introducing a free sulfhydryl group. This functionality is extensively utilised in biochemical research for creating disulfide and thioether linked conjugates, playing a key role in proteomics.
- IUPAC
- 2-Thiolanimine hydrochloride,2IT,Dihydro-2(3H)-thiophenimine hydrochloride,Traut’s reagent
Protein Modification
2-Iminothiolane hydrochloride serves as a thiolating reagent for primary amines in proteins. It enables the introduction of sulfhydryl groups, facilitating subsequent modifications and conjugations.
Protein Structural Analysis
By introducing thiol groups, this reagent aids in the structural elucidation of proteins. It is valuable for studies involving disulfide bond formation or thioether linkages.
Proteomics Research
In proteomics, 2-iminothiolane hydrochloride is employed to modify specific amino acid residues, particularly lysine. This allows for the study of protein interactions and functions.
Conjugate Preparation
The reagent is useful for preparing diverse conjugates, including those linked by disulfide or thioether bonds. It allows for the controlled attachment of thiol functionalities to biomolecules.
| Molecular weight | 137.63 |
|---|---|
| Empirical formula | C4H7NS · HCl |
| Assay | ≥98% (TLC) |
| Form | powder |
| Solubility | H2O: soluble100 mg/mL |
| Storage temperature | 2-8°C |
| Impurities tested | ≤5% Free sulfhydryl groups |
| Melting point | 198-201 °C(lit.) |
| Application | Thiolating reagent for primary amines. Reacts at pH 7-10 by amidine bond to present free sulfhydryl. Useful in preparation of disulfide and/or thioether linked conjugates. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 2-Iminothiolane hydrochloride used for?
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2-Iminothiolane hydrochloride is a thiolating reagent used for the modification of primary amines in proteins. It introduces a free sulfhydryl group, enabling the formation of disulfide and/or thioether linked conjugates, and is valuable in protein structural analysis and proteomics.
What is the CAS number and formula for 2-Iminothiolane hydrochloride?
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The CAS number is 4781-83-3 and the empirical formula is C4H7NS · HCl.
What grade and purity does Tech Serve Solutions supply for 2-Iminothiolane hydrochloride?
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Tech Serve Solutions supplies 2-Iminothiolane hydrochloride with an assay of ≥98% (TLC). We do not represent this product as USP/BP/EP or any other pharmacopoeia grade.
What are the safety and handling precautions for 2-Iminothiolane hydrochloride?
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Appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter, should be used when handling 2-Iminothiolane hydrochloride. It is classified with WGK Germany 3, indicating a high hazard to water.
How is 2-Iminothiolane hydrochloride packed, shipped, and exported?
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2-Iminothiolane hydrochloride is typically stored at 2-8°C. Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals and reagents.
How can I request a quote or sample for 2-Iminothiolane hydrochloride?
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Please contact our sales team directly via our website or by telephone to request a quote or a sample of 2-Iminothiolane hydrochloride.
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