2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt
2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (CAS: 30931-67-0) is a high-purity chemical compound with a molecular weight of 548.68 g/mol. It functions as a sensitive peroxidase substrate, primarily utilised in Enzyme-Linked Immunosorbent Assay (ELISA) procedures. This reagent produces a soluble green end product that can be quantified spectrophotometrically, making it valuable for various biochemical and colorimetric detection applications in research and diagnostics.
- IUPAC
- AzBTS-(NH4)2,Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
- Synonyms
- AzBTS-(NH4)2, Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
ELISA Substrate
This diammonium salt serves as an excellent peroxidase substrate for Enzyme-Linked Immunosorbent Assay (ELISA) procedures. It generates a soluble green-coloured product, which can be accurately measured at 405 nm using spectrophotometry.
Colorimetric Detection
The compound is suitable for various colorimetric detection assays where peroxidase activity needs to be quantified. The formation of a distinct green colour allows for straightforward visual or instrumental analysis.
Biochemical Research
As a core bioreagent, it finds application in fundamental biochemical research. It is particularly useful for enzyme assays involving peroxidases, enabling the study of enzyme kinetics and interactions.
| Molecular weight | 548.68 |
|---|---|
| Empirical formula | C18H24N6O6S4 |
| Application | 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications. |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt used for?
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This compound is primarily used as a peroxidase substrate in Enzyme-Linked Immunosorbent Assay (ELISA) procedures and other colorimetric detection assays where peroxidase activity is measured.
What is the CAS number and formula for this product?
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The CAS number is 30931-67-0 and its empirical formula is C18H24N6O6S4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product as a research-grade chemical. The specific assay or purity is detailed in the product's specifications. We do not represent this product as meeting USP, BP, or EP pharmacopoeia standards.
How should this chemical be handled safely?
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This compound is classified as a warning substance. It may cause skin irritation (H315), serious eye irritation (H319), and respiratory irritation (H335). It is advised to wear appropriate personal protective equipment, including eyeshields, gloves, and a type N95 dust mask, and to avoid breathing dust. Ensure adequate ventilation.
Is this product available for international export?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals, and this product is available for international shipment.
How can I request a quote or sample?
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To request a quote or a sample, please use the contact form on our website or reach out to our sales department directly. We will respond promptly to your inquiry.
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