Demecolcine

CAS 477-30-5C21H25NO5Biochemicals & Reagents

Demecolcine (CAS: 477-30-5; C21H25NO5) is a potent antimitotic agent and a useful biochemical research tool. As a derivative of colchicine, it interferes with microtubule polymerization, arresting cells in metaphase. This property makes it valuable in cell biology research for studying mitosis, chromosome behaviour, and drug-induced cell cycle arrest. It is typically supplied in a ready-to-use concentration in HBSS for convenience in experimental setups.

IUPAC: N-Deacetyl-N-methylcolchicine
Synonyms: N-Deacetyl-N-methylcolchicine

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Demecolcine (C21H25NO5) — chemical structure, CAS 477-30-5; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsEnzyme InhibitorsEnzyme Inhibitors by TypeEnzymesInhibitorsand SubstratesSubstrate Analogs

01 /Applications

Cell Biology Research

Demecolcine is extensively used to investigate the role of microtubules in cellular processes, particularly during cell division. Its ability to arrest cells in metaphase aids in the cytogenetic analysis of chromosomes.

Mitotic Spindle Inhibition

As a potent inhibitor of tubulin polymerization, Demecolcine disrupts the formation of the mitotic spindle. This mechanism is crucial for understanding cancer cell proliferation and developing anti-cancer therapeutics.

Biochemical Reagent

This compound serves as a valuable reagent in biochemical assays that explore protein-drug interactions and enzyme inhibition pathways, particularly those involving microtubule-associated proteins.

02 /Properties

Molecular weight	371.43
Empirical formula	C21H25NO5
Assay	≥98% (HPLC)
Solubility	chloroform: soluble10 mg/mLethanol: soluble
Concentration	10 μg/mL in HBSS
Storage temperature	2-8°C

03 /Safety & handling

GHS hazard pictogram: Toxic (GHS06) — Toxic

Danger

Hazard statements

H300Fatal if swallowed

Precautionary statements

P264Wash thoroughly after handling
P301IF SWALLOWED

Protective equipment	Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
Transport (UN / ADR)	UN 2811 6.1 / PGII
Water hazard class (WGK, DE)	3
Hazard codes (EU)	T
Risk statements (R)	25
Safety statements (S)	22-24/25-45

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

477-30-5

MDL number

MFCD00075459

PubChem substance

24856707

Beilstein registry

2822892

05 /Additional specifications

Sterility	sterile-filtered

06 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

07 /Frequently asked questions

What is Demecolcine used for?

Demecolcine is primarily used in biochemical research as an antimitotic agent to study cell division, chromosome behaviour, and microtubule dynamics. It functions by inhibiting microtubule polymerization and arresting cells in metaphase.

What is the CAS number and formula for Demecolcine?

The CAS number for Demecolcine is 477-30-5, and its molecular formula is C21H25NO5.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Demecolcine with an assay of ≥98% (HPLC). TSS does not represent this product as USP, BP, or EP grade.

How should Demecolcine be handled safely?

Demecolcine is acutely toxic if swallowed (H300) and requires careful handling. Use appropriate personal protective equipment, including eyeshields, faceshields, gloves, and P2 respirator cartridges. In case of accidental ingestion, seek immediate medical attention.

Is Demecolcine supplied sterile?

Yes, Demecolcine is supplied sterile-filtered and is available at a concentration of 10 μg/mL in HBSS.

How is Demecolcine packed, shipped, and exported?

Demecolcine is packed and shipped according to relevant regulations for hazardous materials, including UN 2811 classification for toxic solids. TSS is an experienced global supplier and exporter.