Atto 425 NHS ester
Atto 425 NHS ester (CAS: 892156-28-4, Formula: C26H30N2O8, MW: 498.53 g/mol) is a highly fluorescent labelling agent. Its rigid structure ensures exceptional intensity and minimal spectral shift upon conjugation, making it ideal for high-sensitivity applications, including single-molecule detection. It is readily soluble in DMF, DMSO, and water, offering versatility in experimental setups.
- IUPAC
- Atto 425-N-hydroxysuccinimide ester
Fluorescent Labeling
Atto 425 NHS ester is designed for covalently labelling biomolecules such as proteins and peptides. The NHS ester group reacts readily with primary amines under mild aqueous conditions.
Single Molecule Detection
Due to its high quantum yield and photostability, Atto 425 NHS ester is exceptionally suited for demanding applications like single-molecule fluorescence studies.
Bioconjugation
The compound facilitates the creation of fluorescently tagged biomolecules for various research purposes, leveraging its bright emission at 483 nm (λex 437 nm).
| Molecular weight | 498.53 |
|---|---|
| Empirical formula | C26H30N2O8 |
| Solubility | DMF: solubleDMSO: solubleH2O: soluble |
| Storage temperature | −20°C |
| Application | Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 437 nm; λem 483 nm in 0.1 M phosphate pH 7.0 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Atto 425 NHS ester used for?
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Atto 425 NHS ester is primarily used for fluorescent labelling of biomolecules, especially in high-sensitivity applications such as single-molecule detection, due to its high intensity and minimal spectral shift upon conjugation.
What is the CAS number and formula for Atto 425 NHS ester?
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The CAS number is 892156-28-4, and the empirical formula is C26H30N2O8.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Atto 425 NHS ester as a research grade chemical. We do not represent it as USP, BP, EP, or other pharmacopoeial grades unless explicitly stated in the product specifications.
What are the safety considerations when handling Atto 425 NHS ester?
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When handling Atto 425 NHS ester, it is essential to wear appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. The compound is classified with WGK Germany 3.
How is Atto 425 NHS ester shipped and exported?
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Atto 425 NHS ester is stored at -20°C. Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, capable of handling complex international shipping requirements.
How can I request a sample or quote for Atto 425 NHS ester?
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To request a sample or a quotation for Atto 425 NHS ester, please contact our sales department through the website or by calling our dedicated line.
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