3,3′,5,5′-Tetramethylbenzidine dihydrochloride
3,3′,5,5′-Tetramethylbenzidine dihydrochloride (CAS: 64285-73-0) is a key reagent with the molecular formula C16H20N2 · 2HCl and a molecular weight of 313.27 g/mol. It serves as a sensitive peroxidase substrate, predominantly utilised in enzyme-linked immunosorbent assays (ELISA). Upon enzymatic oxidation, it generates a colourimetric product for accurate detection and quantification in biochemical and diagnostic applications.
- IUPAC
- 4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride
- Synonyms
- 5′-Tetramethylbenzidine dihydrochloride4,4′-Diamino-3,3′,5,5′-tetramethylbiphenyl dihydrochloride
ELISA Substrate
3,3′,5,5′-Tetramethylbenzidine dihydrochloride is a chromogenic substrate for peroxidases in ELISA. It yields a soluble, pale blue end product detectable spectrophotometrically, which turns yellow upon acidification for further analysis.
Colorimetric Detection
This compound facilitates sensitive colorimetric detection in various biochemical assays. Its enzymatic reaction produces a measurable colour change, enabling quantitative analysis of target analytes.
Peroxidase Assays
As a substrate for peroxidase enzymes, it is integral to assays where peroxidase activity needs to be determined. The intensity of the colour produced is proportional to the enzyme's activity.
| Molecular weight | 313.27 |
|---|---|
| Empirical formula | C16H20N2 · 2HCl |
| Form | tablet |
| Storage temperature | 2-8°C |
| Application | 3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. |
| Melting point | ≥300 °C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 3,3′,5,5′-Tetramethylbenzidine dihydrochloride used for?
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3,3′,5,5′-Tetramethylbenzidine dihydrochloride is primarily used as a peroxidase substrate in ELISA and other colorimetric detection assays, producing a measurable colour change upon enzymatic reaction.
What is the CAS number and formula for 3,3′,5,5′-Tetramethylbenzidine dihydrochloride?
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The CAS number is 64285-73-0 and the formula is C16H20N2 · 2HCl.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 3,3′,5,5′-Tetramethylbenzidine dihydrochloride as a technical/research grade reagent. TSS does not represent this product as USP, BP, EP or pharmacopoeia grade.
How should 3,3′,5,5′-Tetramethylbenzidine dihydrochloride be handled safely?
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Handle with appropriate personal protective equipment, including eyeshields and gloves. A respirator filter (type N95 or P1) is recommended. Avoid dust formation and inhalation. It is classified under WGK 2 in Germany.
Is this chemical suitable for export or international shipping?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals. We handle all aspects of packing, shipping, and exporting 3,3′,5,5′-Tetramethylbenzidine dihydrochloride according to international regulations.
How can I request a sample or quote for 3,3′,5,5′-Tetramethylbenzidine dihydrochloride?
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Please contact our sales team directly through the website or by phone to request a sample or a formal quotation for 3,3′,5,5′-Tetramethylbenzidine dihydrochloride.
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