Z-Ile-Glu-Thr-Asp 7-amido-4-trifluoromethylcoumarin

CAS 219138-02-0C37H42F3N5O13Biochemicals & Reagents

Z-Ile-Glu-Thr-Asp 7-amido-4-trifluoromethylcoumarin (CAS: 219138-02-0) is a fluorogenic substrate with the molecular formula C37H42F3N5O13. This peptide derivative is primarily utilised for the detection and quantification of specific enzyme activities. It serves as a valuable tool in biochemical research for studying apoptosis pathways and related cellular processes, enabling precise measurement of enzyme kinetics and substrate cleavage.

IUPAC: Cbz-IETD-AFC

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Z-Ile-Glu-Thr-Asp 7-amido-4-trifluoromethylcoumarin (C37H42F3N5O13) — chemical structure, CAS 219138-02-0; Life Science, fine chemical supplied by Tech Serve Solutions

Apoptosis EnzymesApoptosis and Cell CycleApplication IndexBioactive Small MoleculesBiochemicals and ReagentsCaspase 8Caspase SubstratesCaspasesCell BiologyCell Signaling Enzymes

01 /Applications

Fluorogenic Substrate for Caspase 8

This compound acts as a fluorogenic substrate specifically designed for the activity assay of Caspase 8. Upon enzymatic cleavage by Caspase 8, it releases a fluorescent signal, allowing for quantitative measurement of enzyme activity in biological samples.

Assay for Granzyme B Activity

Z-Ile-Glu-Thr-Asp 7-amido-4-trifluoromethylcoumarin is also employed as a substrate to detect and measure the activity of Granzyme B. The cleavage of this substrate by Granzyme B results in a measurable fluorescent output, facilitating research into cell-mediated cytotoxicity.

Apoptosis Research

As a substrate for key apoptotic enzymes like Caspase 8, this chemical is instrumental in studying the biochemical mechanisms of programmed cell death. Researchers use it to investigate signaling cascades involved in apoptosis.

Cell Signaling Studies

The compound aids in understanding cell signaling pathways, particularly those involving cysteine proteases. Its use allows for the monitoring of enzyme activity that plays a role in various cellular functions and disease states.

02 /Properties

Molecular weight	821.75
Empirical formula	C37H42F3N5O13
Assay	≥95% (HPLC)
Storage temperature	−20°C
Application	Fluorogenic substrate for caspase 8 and granzyme B.

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

219138-02-0

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is Z-Ile-Glu-Thr-Asp 7-amido-4-trifluoromethylcoumarin used for?

Z-Ile-Glu-Thr-Asp 7-amido-4-trifluoromethylcoumarin is used as a fluorogenic substrate for assaying the activity of Caspase 8 and Granzyme B, making it valuable for research in apoptosis and cell signaling.

What are the CAS number and molecular formula?

The CAS number for Z-Ile-Glu-Thr-Asp 7-amido-4-trifluoromethylcoumarin is 219138-02-0, and its molecular formula is C37H42F3N5O13.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies this product with an assay of ≥95% (HPLC). We do not represent this product as USP, BP, EP, or pharmaceutical grade.

How should this chemical be handled safely?

Handle with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if necessary. The product has a WGK Germany rating of 3, indicating it is hazardous to water.

How is this product shipped?

This product is shipped and exported by Tech Serve Solutions under standard conditions for fine chemicals, typically requiring storage at −20°C.

How can I request a quote or sample?

To request a quote or sample, please contact our sales team via the contact form on our website or call us directly. Specify the product name and CAS number.