Z-Gly-Pro-4-nitroanilide
Z-Gly-Pro-4-nitroanilide (CAS 65022-15-3), with the empirical formula C21H22N4O6 and a molecular weight of 426.42 g/mol, is a biochemical reagent. It serves as a chromogenic substrate, primarily for the enzymatic determination of prolyl endopeptidase activity. This compound is essential in biochemical research and diagnostics for quantifying enzyme kinetics and specificity.
- IUPAC
- Z-glycyl-L-proline-4-nitroanilide

Enzyme Activity Assays
Used as a chromogenic substrate to quantify the activity of enzymes, particularly prolyl endopeptidases. The release of 4-nitroaniline upon enzymatic cleavage provides a quantifiable spectrophotometric signal.
Biochemical Research
Facilitates studies into enzyme mechanisms, kinetics, and inhibitor screening in various biological systems. Its specific cleavage by certain peptidases makes it a valuable tool for biochemical investigations.
Diagnostic Applications
Can be employed in diagnostic kits for the detection and quantification of specific enzyme levels in biological samples, aiding in disease research and understanding.
| Molecular weight | 426.42 |
|---|---|
| Empirical formula | C21H22N4O6 |
| Assay | ≥99.0% (TLC) |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Z-Gly-Pro-4-nitroanilide primarily used for?
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Z-Gly-Pro-4-nitroanilide is primarily used as a chromogenic substrate for the enzymatic determination of prolyl endopeptidase activity, essential in biochemical research and diagnostics.
What is the CAS number and molecular formula for Z-Gly-Pro-4-nitroanilide?
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The CAS number is 65022-15-3, and the molecular formula is C21H22N4O6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Z-Gly-Pro-4-nitroanilide with a purity of ≥99.0% (TLC). This product is supplied for research and laboratory use and is not represented as USP, BP, EP, or pharmacopoeia grade.
How should Z-Gly-Pro-4-nitroanilide be handled and stored?
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Handle Z-Gly-Pro-4-nitroanilide with appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. Store at -20°C.
Is Z-Gly-Pro-4-nitroanilide a controlled substance?
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The provided data does not indicate that Z-Gly-Pro-4-nitroanilide is a controlled substance.
How can I request a quote or sample?
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To request a quote or sample of Z-Gly-Pro-4-nitroanilide, please contact our sales team through the website or by phone.
How is Z-Gly-Pro-4-nitroanilide packed, shipped, and exported?
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Z-Gly-Pro-4-nitroanilide is carefully packed according to industry standards for safe transport. Tech Serve Solutions is a global supplier and exporter, capable of shipping to numerous international destinations.
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