Tris(hydroxymethyl)aminomethane
Tris(hydroxymethyl)aminomethane (CAS: 77-86-1; Formula: NH2C(CH2OH)3) is a white crystalline solid with a molecular weight of 121.14 g/mol. This versatile compound functions as a primary amine and a primary alcohol, making it valuable in various chemical syntheses and biological applications. It is widely recognised for its buffering capabilities within a specific pH range, commonly employed in biochemical research and diagnostics.
- IUPAC
- 2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Tris(hydroxymethyl)aminomethane,Trometamol,Trometamolum
- Synonyms
- 2-Amino-2-(hydroxymethyl)-1,3-propanediolTHAMTris baseTrometamolTrometamolum
Biochemical Buffering
Tris is a fundamental buffering agent in molecular biology and biochemistry, maintaining stable pH levels (typically pH 7-9) crucial for enzymatic reactions, protein purification, and electrophoresis.
Pharmaceutical Intermediate
Its structural features allow it to serve as a building block or intermediate in the synthesis of various pharmaceutical compounds, contributing to the development of new therapeutic agents.
Diagnostic Reagent
In diagnostic kits and assays, Tris ensures consistent reaction conditions, contributing to the reliability and accuracy of results in clinical and research settings.
Chemical Synthesis
As an organic building block, Tris(hydroxymethyl)aminomethane participates in diverse chemical reactions, enabling the creation of complex molecules for research and industrial applications.
| Molecular weight | 121.14 |
|---|---|
| Linear formula | NH2C(CH2OH)3 |
| Assay | ≥99.8% |
| Grade | ACS reagent |
| Boiling point | 219-220 °C/10 mmHg(lit.) |
| Solubility | water: soluble678 g/L at 20 °C |
| pH | 10.5-12.0 (25 °C, 4 M in H2O) |
| Melting point | 167-172 °C(lit.) |
| Loss on drying | ≤0.5% loss on drying, 110 °C |
| Impurities tested | ≤0.2% water (Karl Fischer) |
| Suitability | in accordance for luminescence |
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 2 |
| Cation traces | heavy metals (as Pb): ≤2 ppm |
| Anion traces | chloride (Cl-): ≤20 mg/kgsulfate (SO42-): ≤5 mg/kg |
| Ignition residue | ≤0.01% (as SO4) |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Pka 25 °C | 8.1 |
|---|---|
| Uv Absorption | λ: 260 nm Amax: 0.10λ: 280 nm Amax: 0.08 |
| Absorption | A40%/290 nm ≤0.05 |
| Ph Range | 7 - 9 |
| Useful Ph Range | 7 - 9 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Tris(hydroxymethyl)aminomethane used for?
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Tris(hydroxymethyl)aminomethane (Tris) is primarily used as a biological buffer in molecular biology, biochemistry, and diagnostics due to its effective buffering capacity in the pH range of 7-9. It also serves as an intermediate and building block in chemical synthesis.
What is the CAS number and formula for Tris(hydroxymethyl)aminomethane?
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The CAS number is 77-86-1, and the chemical formula is NH2C(CH2OH)3.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Tris(hydroxymethyl)aminomethane in ACS reagent grade with an assay of ≥99.8%.
How should Tris(hydroxymethyl)aminomethane be handled safely?
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When handling Tris(hydroxymethyl)aminomethane, wear appropriate personal protective equipment, including a dust mask type N95 (US), eyeshields, and gloves. Avoid dust formation and ensure good ventilation.
Is Tris(hydroxymethyl)aminomethane a controlled substance?
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Based on the provided data, Tris(hydroxymethyl)aminomethane is not indicated as a controlled substance.
How is Tris(hydroxymethyl)aminomethane packed and exported?
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Tech Serve Solutions, as a global supplier, exports chemicals like Tris(hydroxymethyl)aminomethane in compliance with international shipping regulations.
How can I request a sample or quote for Tris(hydroxymethyl)aminomethane?
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To request a sample or a quote, please contact our sales department through the website or by phone, providing the product name and CAS number.
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