Sudan Black B
Sudan Black B (CAS 4197-25-5, C29H24N6) is a black synthetic organic dye with a molecular weight of 456.54 g/mol. It is known for its ability to stain lipids and is widely employed as a stain and dye in various biological and histological applications. This compound is valued for its effectiveness in visualizing lipid-rich structures within cells and tissues.
- IUPAC
- Ceres black BN,Fat Black HB,Solvent Black 3
- Synonyms
- Ceres black BNFat Black HBSolvent Black 3Sudan BlackSudan Black B dyeOil Black B

Lipid Staining in Cell Biology
Sudan Black B is a key lipophilic dye used to stain triglycerides and other lipids within cells. It enables researchers to identify and study lipid droplets and their distribution in various biological samples.
Hematology and Histology Staining
This dye is extensively used in hematology and histology for staining blood cells and tissue sections. It is particularly effective for demonstrating neutral lipids in myeloid cells and can be used in cytochemistry.
Enzyme Histochemistry
Sudan Black B can be employed in enzyme histochemistry to stain the products of certain enzyme activities, especially those involving lipids, providing visual localisation of enzymatic processes within tissues.
| Molecular weight | 456.54 |
|---|---|
| Empirical formula | C29H24N6 |
| Melting point | 120-124 °C(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 26150 |
|---|---|
| Absorption | λmax 415 nm (2nd)λmax 598 nmextinction/596-603 nm, ethanol ≥20000 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Sudan Black B used for?
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Sudan Black B is primarily used as a lipophilic stain in cell biology, hematology, and histology for the visualisation of lipids and lipid-rich structures in cells and tissues.
What are the CAS number and formula for Sudan Black B?
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The CAS number for Sudan Black B is 4197-25-5, and its molecular formula is C29H24N6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Sudan Black B as a research-grade chemical. We do not represent it as USP, BP, EP, or for any pharmacopoeia, nutraceutical, or food applications. Purity information is available on the Certificate of Analysis.
What are the safety precautions for handling Sudan Black B?
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Handle Sudan Black B with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Consult the Safety Data Sheet for full handling and hazard information.
How is Sudan Black B packed and shipped?
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Sudan Black B is carefully packed to ensure safe transit and is exported globally by Tech Serve Solutions in compliance with international shipping regulations for chemicals.
How can I request a sample or quote for Sudan Black B?
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To request a sample or a quote for Sudan Black B, please contact our sales department through the website or by calling us directly.
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