Sudan III
Sudan III (CAS: 85-86-9), with the molecular formula C22H16N4O and a molecular weight of 352.39 g/mol, is a lipophilic azo dye. It is primarily utilised as a biological stain in histology and cell biology for the differential staining of lipids, fats, and oils. Its vibrant red colour makes it useful for visualising these components in tissue samples, aiding in microscopic examination and research.
- IUPAC
- 1-[4-(Phenylazo)phenylazo]-2-naphthol,Cerasin Red,Fat Ponceau G,Fat Soluble Sudan,Scarlet B,Solvent Red 23,Sudan G,Sudan Red BK,Tony Red
- Synonyms
- 1-[4-(Phenylazo)phenylazo]-2-naphtholCerasin RedFat Ponceau GFat Soluble SudanScarlet BSolvent Red 23Sudan GSudan Red BKTony Red

Lipid Staining in Histology
Sudan III is widely employed in histological techniques to stain triglycerides and other lipids within tissue sections. This allows for the identification and localisation of fatty tissues and cellular lipid deposits under microscopic examination.
Cell Biology Research
In cell biology, Sudan III serves as a vital stain for visualising lipid droplets and other fat-soluble substances within cells. This application supports research into cellular metabolism and lipid dynamics.
Diagnostic Staining
The dye can be used in diagnostic pathology to highlight the presence and distribution of fats, which can be indicative of certain pathological conditions. It aids in the microscopic assessment of tissue health.
| Molecular weight | 352.39 |
|---|---|
| Empirical formula | C22H16N4O |
| Melting point | 199 °C (dec.)(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 26100 |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Sudan III used for?
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Sudan III is primarily used as a biological stain in histology and cell biology for the differential staining of lipids, fats, and oils, making them visible under a microscope.
What is the CAS number and molecular formula for Sudan III?
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The CAS number for Sudan III is 85-86-9, and its molecular formula is C22H16N4O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Sudan III as a technical/research grade chemical. We do not represent this product as USP, BP, or EP grade.
What are the safety precautions for handling Sudan III?
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Sudan III is classified with GHS hazards. When handling, it is essential to wear appropriate personal protective equipment, including eyeshields and gloves, and use a P1 or N95 respirator filter. Refer to the safety data sheet for comprehensive handling guidelines.
How is Sudan III packaged and shipped?
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Sudan III is carefully packaged to maintain its integrity during transit and is exported by Tech Serve Solutions adhering to all relevant shipping regulations for chemical substances.
How can I request a sample or quote for Sudan III?
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To request a sample or a quote for Sudan III, please contact our sales team through the provided channels on the Tech Serve Solutions website.
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