Resorufin β-D-glucopyranoside
Resorufin β-D-glucopyranoside (CAS: 101490-85-1; Formula: C18H17NO8; Molar Mass: 375.33 g/mol) is a valuable biochemical reagent. This fluorogenic substrate is particularly useful for detecting enzyme activity, primarily for β-glucosidase. Its ability to release the fluorescent resorufin upon enzymatic cleavage makes it an essential tool in various biochemical assays and molecular biology applications for quantifying enzyme kinetics and screening inhibitors.
- IUPAC
- 3-Phenoxazone 7-(β-D-glucopyranoside)
Enzyme Activity Assays
Resorufin β-D-glucopyranoside serves as a sensitive fluorogenic substrate for the quantitative determination of β-glucosidase activity. Upon enzymatic hydrolysis, it releases resorufin, a highly fluorescent molecule, allowing for real-time monitoring of enzyme kinetics and reaction rates.
Drug Discovery and Screening
This compound is instrumental in high-throughput screening (HTS) campaigns for identifying enzyme inhibitors. By measuring changes in resorufin fluorescence, researchers can efficiently screen large libraries of potential drug candidates that modulate β-glucosidase activity.
Molecular Biology Research
In molecular biology, Resorufin β-D-glucopyranoside is employed in studies involving gene expression and enzyme function. Its fluorescent readout facilitates sensitive detection and quantification in various experimental setups.
Biochemical Research
As a specialized biochemical reagent, it aids in understanding glycosidase mechanisms and cellular processes involving glucoside hydrolysis. Its application spans across various fields of biochemical research requiring precise enzymatic activity measurements.
| Molecular weight | 375.33 |
|---|---|
| Empirical formula | C18H17NO8 |
| Assay | ≥95% (HPLC) |
| Solubility | DMF: solubleDMSO: solubleH2O: soluble |
| Storage temperature | −20°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 571 nm; λem 590 nm in 0.1 M phosphate pH 7.0 (β-glucosidase) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Resorufin β-D-glucopyranoside used for?
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Resorufin β-D-glucopyranoside is a fluorogenic substrate primarily used for the detection and quantification of β-glucosidase activity in biochemical assays, drug discovery, and molecular biology research.
What are the CAS number and formula for Resorufin β-D-glucopyranoside?
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The CAS number is 101490-85-1 and the empirical formula is C18H17NO8.
What grade and purity does Tech Serve Solutions supply for Resorufin β-D-glucopyranoside?
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Tech Serve Solutions supplies Resorufin β-D-glucopyranoside with an assay of ≥95% (HPLC). We do not represent this product as USP, BP, EP, or pharmaceutical grade.
What are the safety considerations when handling Resorufin β-D-glucopyranoside?
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According to safety data, appropriate personal protective equipment including eyeshields and gloves should be worn. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The compound is classified under WGK Germany 3, indicating a high hazard to water.
How is Resorufin β-D-glucopyranoside shipped and exported by TSS?
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Tech Serve Solutions, as a specialist global supplier and exporter, ensures appropriate packaging and handling for the export of chemicals like Resorufin β-D-glucopyranoside, adhering to international shipping regulations.
How can I request a quote or sample for Resorufin β-D-glucopyranoside?
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To request a quote or a sample of Resorufin β-D-glucopyranoside, please contact our sales department through the website or by phone.
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