Puromycin dihydrochloride
Puromycin dihydrochloride (CAS: 58-58-2) is an antibacterial and antibiotic compound isolated from *Streptomyces alboniger*. It functions by inhibiting protein synthesis, making it a valuable tool in molecular biology and cell culture research for selection. It is also utilized in studies involving cell viability and the development of transgenic organisms. Tech Serve Solutions supplies this PREMIUM grade reagent for research applications.
Cell Viability Studies
Puromycin dihydrochloride can be used to assess cell viability, particularly in studies involving vascular smooth muscle cells, as demonstrated in rabbit models.
Protein Synthesis Inhibition
As an antibiotic, puromycin dihydrochloride effectively inhibits protein synthesis in cells, making it useful for selection protocols when used in conjunction with resistance genes like puromycin N-acetyl-transferase (PAC).
Biotechnology Research
This compound has been applied in advanced biotechnological research, such as the creation of enhanced green fluorescent protein (EGFP) transgenic piglets via somatic cell cloning and embryo transfer.
| Assay | >98% (HPLC) |
|---|---|
| Solubility | H2O: soluble50 mg/mL (Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C.) |
| Concentration | 10 mg/mL in H2O |
| Storage temperature | −20°C |
| Application | Puromycin is an aminonucleoside antibiotic that is derived from Streptomyces alboniger. It allows selection for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). It has been used to study vascular smooth muscle cell viability after treatment in the rabbit model 1. Puromycin is used to produce enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer2. |
| Suitability | cell culture tested |
| Biological source | from Streptomyces alboniger |
| Protective equipment | Eyeshields, Faceshields, Gloves, half-mask respirator (EU), half-mask respirator (US), multi-purpose combination respirator cartridge (US) |
|---|---|
| Water hazard class (WGK, DE) | 2 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Quality Level | PREMIUM |
|---|---|
| Shipped In | wet ice |
| Sterility | 0.2 μm filtered |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Puromycin dihydrochloride used for?
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Puromycin dihydrochloride is primarily used for its antibacterial properties, to inhibit protein synthesis in cells, and as a selection agent in cell culture when the puromycin resistance gene is present.
What is the CAS number and molecular formula for Puromycin dihydrochloride?
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The CAS number for Puromycin dihydrochloride is 58-58-2. The molecular formula is not provided in the data.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Puromycin dihydrochloride at PREMIUM grade with an assay of >98% (HPLC). It is tested for suitability in cell culture.
What are the safety and handling precautions for Puromycin dihydrochloride?
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When handling Puromycin dihydrochloride, appropriate personal protective equipment including eyeshields, faceshields, gloves, and respiratory protection (half-mask respirator) should be used. Refer to the safety data sheet for full hazard information. It has a WGK Germany rating of 2.
How is Puromycin dihydrochloride shipped and stored?
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Puromycin dihydrochloride is shipped in wet ice and should be stored at -20°C. Stock solutions are prepared at 10 mg/mL in H2O and are 0.2 μm filtered for sterility.
How can I request a quote or sample for Puromycin dihydrochloride?
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To request a quote or sample for Puromycin dihydrochloride, please contact our sales team via the website or listed phone number.
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