Propidium iodide
Propidium iodide (CAS: 25535-16-4, C27H34I2N4) is a fluorescent intercalating agent with a molecular weight of 668.39 g/mol. It is commonly used as a vital stain in cell biology for identifying dead cells. This compound emits red fluorescence when excited by blue light, making it a valuable tool for flow cytometry and microscopy in research settings. It is supplied by Tech Serve Solutions for laboratory use.
- IUPAC
- 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
- Synonyms
- 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
Cell Viability Staining
Propidium iodide is a widely used fluorescent stain to differentiate between viable and non-viable cells. It can only enter cells with damaged cell membranes, characteristic of late-stage apoptosis or necrosis.
Flow Cytometry
Its ability to intercalate into DNA and produce red fluorescence upon excitation makes it an essential reagent in flow cytometry for assessing cell death and cell cycle analysis.
Fluorescence Microscopy
In fluorescence microscopy, propidium iodide serves to visualise dead or dying cells within a population, aiding in the study of cellular processes and the effects of treatments.
Histology and Hematology
This stain is also applicable in histological preparations and hematological studies for distinguishing cell viability in tissue samples and blood cells.
| Molecular weight | 668.39 |
|---|---|
| Empirical formula | C27H34I2N4 |
| Assay | ≥94.0% (HPLC) |
| Concentration | 1 mg/mL in H2O |
| Storage temperature | 2-8°C |
| Melting point | 220-225 °C (dec.)(lit.) |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
- H341Suspected of causing genetic defects
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P281Use personal protective equipment as required
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 36/37/38-68 |
| Safety statements (S) | 26-36/37 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 530 nm; λem 620 nm in 0.1 M phosphate pH 7.0 (bound to DNA) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Propidium iodide primarily used for?
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Propidium iodide (CAS: 25535-16-4, C27H34I2N4) is primarily used as a fluorescent stain to identify cells with compromised cell membranes, typically indicating cell death, in research applications such as flow cytometry and microscopy.
What are the CAS number and formula for Propidium iodide?
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The CAS number for Propidium iodide is 25535-16-4, and its empirical formula is C27H34I2N4.
What grade and purity does Tech Serve Solutions supply for Propidium iodide?
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Tech Serve Solutions supplies Propidium iodide with an assay of ≥94.0% (HPLC). TSS does not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
What are the safety and handling considerations for Propidium iodide?
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Propidium iodide is a Warning substance (GHS07, GHS08) with hazards H315, H319, H335, and H341, indicating it may cause skin irritation, serious eye irritation, respiratory irritation, and is suspected of causing genetic defects. Appropriate personal protective equipment including eyeshields, gloves, and a dust mask (N95 US) should be used. Handle in a well-ventilated area and avoid inhalation of dust.
How is Propidium iodide packed, shipped, and exported by TSS?
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Propidium iodide is stored at 2-8°C and is available in a concentration of 1 mg/mL in H2O. We adhere to all regulations for packing, shipping, and exporting fine chemicals globally.
How can I request a sample or quote for Propidium iodide?
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To request a sample or a quote for Propidium iodide, please contact our sales team through the website or via email, providing your specific requirements.
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