Pro-Phe-Arg-7-amido-4-methylcoumarin
Pro-Phe-Arg-7-amido-4-methylcoumarin (CAS 65147-21-9) is a synthetic peptide derivative with the molecular formula C30H37N7O5. This fluorogenic substrate is primarily used in biochemical research for the detection and quantification of specific enzyme activities. Its reliable performance makes it a valuable tool in studies involving proteases and peptidases, offering a fluorescent signal upon enzymatic cleavage.

Enzyme Activity Assays
Utilised as a fluorogenic substrate for the detection of specific protease and peptidase activities. Enzymatic cleavage releases a fluorescent moiety, enabling quantitative measurements.
Biochemical Research
A key reagent in molecular biology and biochemistry laboratories for investigating enzyme kinetics and mechanisms. Its structure allows for specific substrate recognition by certain enzymes.
Protease Profiling
Aids in the characterisation and profiling of protease families. The compound facilitates the identification of enzyme specificity and potential inhibitors.
| Molecular weight | 575.66 |
|---|---|
| Empirical formula | C30H37N7O5 |
| Assay | ≥95% |
| Storage temperature | −20°C |
| Composition | Peptide content, ≥65% |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Pro-Phe-Arg-7-amido-4-methylcoumarin used for?
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This compound is used as a fluorogenic substrate for detecting and quantifying specific enzyme activities, particularly in biochemical and molecular biology research involving proteases and peptidases.
What are the CAS number and formula for Pro-Phe-Arg-7-amido-4-methylcoumarin?
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The CAS number is 65147-21-9 and the molecular formula is C30H37N7O5.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Pro-Phe-Arg-7-amido-4-methylcoumarin with an assay of ≥95%. We do not represent this product as USP, BP, or EP grade.
What are the safety and handling recommendations?
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Appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or P1 (EN143) respirator filter, should be used. The product has a WGK rating of 3 in Germany, indicating a high hazard to water.
How is this chemical shipped and exported?
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Tech Serve Solutions is an experienced global supplier and exporter of fine chemicals, ensuring appropriate packaging and documentation for international shipments.
How can I request a sample or quote?
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Please contact our sales team via the website or directly to request a sample or a quotation for Pro-Phe-Arg-7-amido-4-methylcoumarin.
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