PIPES
Piperazine-1,4-bis(2-ethanesulfonic acid) (CAS: 5625-37-6), with the molecular formula C8H18N2O6S2 and a molecular weight of 302.37 g/mol, is a biological buffer. This high-purity grade chemical is suitable for demanding applications in molecular biology, cell culture, and manufacturing processes where consistent pH control is critical. Its robust buffering capacity ensures stability across a useful pH range.
- IUPAC
- 1,4-Piperazinediethanesulfonic acid,Piperazine-1,4-bis(2-ethanesulfonic acid),Piperazine-N,N′-bis(2-ethanesulfonic acid)
- Synonyms
- 1,4-Piperazinediethanesulfonic acidPiperazine-1,4-bis(2-ethanesulfonic acid)Piperazine-N,N′-bis(2-ethanesulfonic acid)
Molecular Biology Research
This buffer is employed in molecular biology techniques requiring precise pH control and minimal interference. Its low UV absorbance and absence of detectable nucleases ensure reliable results in sensitive assays.
Cell Culture Media
The product's suitability for cell culture makes it an ideal component for maintaining stable pH in growth media. Its formulation is designed to support cellular viability and optimal growth conditions.
Biochemical Assays
Utilised in various biochemical assays, this buffer component helps maintain physiological pH conditions. Its high purity and defined specifications minimise the risk of experimental artefacts.
Manufacturing Processes
With suitability for manufacturing use, this buffer is incorporated into industrial biochemical processes. Its consistent quality and performance are essential for reproducible large-scale production.
| Molecular weight | 302.37 |
|---|---|
| Empirical formula | C8H18N2O6S2 |
| Assay | ≥99.5% (T) |
| Grade | for molecular biology |
| Solubility | 0.1 M NaOH: soluble0.1 M at 20 °C, clear, colorless |
| Loss on drying | ≤0.2% loss on drying, 110 °C |
| Impurities tested | DNases, none detectedRNases, none detectedinsoluble matter, passes filter testphosphatases, none detectedproteases, none detected |
| Melting point | >300 °C(lit.) |
| Suitability | suitable for cell culturesuitable for manufacturing use |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Anion traces | chloride (Cl-): ≤200 mg/kgsulfate (SO42-): ≤50 mg/kg |
| Ignition residue | ≤0.2% (as SO4) |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioXtra |
|---|---|
| Pka 25 °C | 6.8 |
| λ | 0.1 M in 1 M NaOH |
| Uv Absorption | λ: 260 nm Amax: 0.055λ: 280 nm Amax: 0.040 |
| Useful Ph Range | 6.1 - 7.5 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is PIPES used for?
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PIPES is primarily used as a biological buffer, particularly in molecular biology, cell culture, and biochemical assays, owing to its effective buffering capacity within the pH range of 6.1-7.5 and its suitability for manufacturing use.
What is the CAS number and formula for PIPES?
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The CAS number for PIPES is 5625-37-6, and its empirical formula is C8H18N2O6S2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies PIPES in a grade suitable for molecular biology, with an assay of ≥99.5% (T) and DNases, RNases, phosphatases, and proteases detected as none.
How should PIPES be handled safely?
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When handling PIPES, it is recommended to use safety equipment including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter may be necessary. Refer to the Safety Data Sheet for comprehensive handling information.
Is PIPES a controlled substance?
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The provided data does not indicate that PIPES is a controlled substance.
How is PIPES packed and exported?
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PIPES is available from Tech Serve Solutions, a global supplier and exporter. Specific packing and shipping details for export can be provided upon request.
How can I request a sample or quote for PIPES?
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To request a sample or a quote for PIPES, please contact our sales team through the website or direct inquiry channels.
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