Phloxine B
Phloxine B (CAS 18472-87-2), with the molecular formula C20H2Br4Cl4Na2O5, is a synthetic organic compound recognised for its vibrant red colour. It functions as a vital stain in biological and histological applications, particularly for staining cytoplasmic material and collagen. Its specific chemical structure allows for precise cellular visualization, making it an indispensable reagent in research and diagnostic laboratories.
- IUPAC
- 2′,4′,5′,7′-Tetrabromo-4,5,6,7-tetrachlorofluorescein disodium salt,Acid Red 92,Cyanosine,D&C;Red No. 28,Eosin 10B
- Synonyms
- 2′,4′,5′,7′-Tetrabromo-4,5,6,7-tetrachlorofluorescein disodium saltAcid Red 92CyanosineD&C Red No. 28Eosin 10B

Histology and Cytology Staining
Phloxine B is extensively used as a counterstain in histological and cytological preparations. It effectively stains cytoplasm, collagen, and other extracellular components a distinct pink to red colour, aiding in the differentiation of tissue structures.
Cell Biology Research
In cell biology, Phloxine B serves as a vital stain to assess cell viability and membrane integrity. Its ability to penetrate damaged cell membranes allows researchers to distinguish between live and dead cells in experimental assays.
Microscopy Reagent
As a certified stain with Colour Index Number 45410, Phloxine B is a crucial component in various microscopy techniques. It provides excellent contrast and differential staining, enhancing the visualization of cellular and tissue morphology.
| Molecular weight | 829.63 |
|---|---|
| Empirical formula | C20H2Br4Cl4Na2O5 |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 2 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 45410 |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Phloxine B used for?
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Phloxine B is primarily used as a histological and cytological stain, especially for staining cytoplasmic material and collagen. It is also utilized in cell biology to assess cell viability and in microscopy for differential staining of tissues.
What are the CAS number and formula for Phloxine B?
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The CAS number for Phloxine B is 18472-87-2, and its molecular formula is C20H2Br4Cl4Na2O5.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Phloxine B as a high-quality reagent suitable for research and laboratory use. The precise assay and grade are detailed on the product's Certificate of Analysis. TSS does not represent this product as USP, BP, or EP grade unless explicitly stated.
How should Phloxine B be handled safely?
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Phloxine B should be handled with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if airborne exposure is possible. Refer to the Safety Data Sheet for detailed handling and hazard information.
Is Phloxine B a controlled substance?
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The provided data does not indicate that Phloxine B (CAS 18472-87-2) is a controlled substance.
How is Phloxine B packed and shipped?
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Phloxine B is carefully packed according to international shipping regulations for chemicals. Tech Serve Solutions ensures secure packaging for safe export to destinations worldwide.
How can I request a quote or sample for Phloxine B?
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To request a quote or a sample of Phloxine B, please visit the Tech Serve Solutions website or contact our sales department directly with your specific requirements.
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