N-Succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin
N-Succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin (CAS 71973-79-0) is a fluorogenic substrate with the molecular formula C29H32N4O8 and a molecular weight of 564.59 g/mol. It is primarily utilised in biochemical research for the detection and quantification of specific enzyme activities. This compound serves as a valuable tool for studying proteases and other enzymes that cleave succinyl-Ala-Ala-Phe peptide bonds, leading to a fluorescent signal.

Enzyme Activity Assays
N-Succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin functions as a sensitive fluorogenic substrate. Its cleavage by specific enzymes releases a fluorescent moiety, enabling the precise measurement of enzyme kinetics and activity in various biological samples.
Protease Research
This compound is employed in research settings to investigate the function and inhibition of proteases. It aids in the identification of enzymes that recognise and process the succinyl-Ala-Ala-Phe sequence within complex biological systems.
Biochemical Studies
Utilised as a reagent in biochemical investigations, it assists in understanding enzyme mechanisms and screening for enzyme modulators. The fluorescence generated upon enzymatic hydrolysis allows for high-throughput screening applications.
| Molecular weight | 564.59 |
|---|---|
| Empirical formula | C29H32N4O8 |
| Storage temperature | −20°C |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is N-Succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin used for?
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N-Succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin is used as a fluorogenic substrate for detecting and quantifying enzyme activity, particularly proteases that cleave succinyl-Ala-Ala-Phe peptide bonds.
What is the CAS number and formula for N-Succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin?
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The CAS number is 71973-79-0 and the empirical formula is C29H32N4O8.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this compound as a research-grade chemical. The specific assay and purity are detailed in the product's Certificate of Analysis. It is not supplied as USP, BP, or EP grade.
How should N-Succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin be handled?
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Handle with appropriate personal protective equipment, including a dust mask (type N95 recommended), eyeshields, and gloves. Ensure adequate ventilation, avoiding dust formation and inhalation. Refer to the Safety Data Sheet for comprehensive handling instructions.
How is N-Succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin shipped?
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This chemical is shipped and exported under conditions suitable for its stability, typically requiring storage at -20°C. Consult our shipping department for specific international export regulations.
How can I request a sample or quote for N-Succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin?
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To request a sample or a quotation, please contact our sales team through the website or by phone, providing your specific requirements and shipping details.
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