N-Succinyl-Ala-Ala-Ala-p-nitroanilide

CAS 52299-14-6C19H25N5O8Biochemicals & Reagents

N-Succinyl-Ala-Ala-Ala-p-nitroanilide (CAS 52299-14-6) is a synthetic peptide derivative with the molecular formula C19H25N5O8 and a molecular weight of 451.43 g/mol. This compound serves as a chromogenic substrate for the enzymatic determination of proteases, particularly elastase. Its predictable cleavage allows for quantitative assays in biochemical research and diagnostics.

IUPAC: N-Succinyl-L-alanyl-L-alanyl-L-alanine 4-nitroanilide,N-Succinyl-tri-L-alanine 4-nitroanilide

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N-Succinyl-Ala-Ala-Ala-p-nitroanilide (C19H25N5O8) — chemical structure, CAS 52299-14-6; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsCell BiologyElastaseEnzyme SubstratesEnzymesInhibitorsand SubstratesPeptides and ProteinsPeptides for Cell BiologyProtease Substrates

01 /Applications

Enzyme Substrate for Protease Assays

N-Succinyl-Ala-Ala-Ala-p-nitroanilide is widely used as a chromogenic substrate for assaying elastase activity. Upon enzymatic cleavage, it releases p-nitroaniline, which can be quantified spectrophotometrically, enabling the measurement of enzyme kinetics and inhibitor efficacy.

Biochemical Research Tool

This peptide derivative is valuable in cell biology and cell signaling research for studying protease function. It assists in the investigation of enzymes involved in processes like protein degradation and cellular communication pathways.

Diagnostic Reagent Development

Its utility as a specific substrate makes it a candidate for developing diagnostic assays that detect or quantify protease activity relevant to various physiological and pathological conditions.

02 /Properties

Molecular weight	451.43
Empirical formula	C19H25N5O8
Storage temperature	2-8°C

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

52299-14-6

MDL number

MFCD00036774

PubChem substance

24899617

Beilstein registry

2928351

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is N-Succinyl-Ala-Ala-Ala-p-nitroanilide used for?

It is primarily used as a chromogenic substrate for the assay of protease activity, especially elastase. The release of p-nitroaniline upon cleavage allows for quantitative measurement of enzyme activity.

What are the CAS number and formula for N-Succinyl-Ala-Ala-Ala-p-nitroanilide?

The CAS number is 52299-14-6, and its molecular formula is C19H25N5O8.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies N-Succinyl-Ala-Ala-Ala-p-nitroanilide as a research-grade chemical. Specific purity assays are available upon request, and TSS does not represent this product as USP, BP, EP, or pharmaceutical grade.

How should N-Succinyl-Ala-Ala-Ala-p-nitroanilide be handled safely?

Handling requires appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. The compound is classified under WGK Germany 3.

How is N-Succinyl-Ala-Ala-Ala-p-nitroanilide packed and shipped?

This product is typically stored at 2-8°C. TSS handles the packing, shipping, and export of chemicals according to international regulations.

How can I request a sample or quote for N-Succinyl-Ala-Ala-Ala-p-nitroanilide?

To request a sample or a quote, please contact our sales team through the website or by phone, providing the product name and CAS number (52299-14-6).