N,N′-Hexamethylene bis(acetamide)
N,N′-Hexamethylene bis(acetamide) (CAS: 3073-59-4) is a chemical compound with the linear formula CH₃CONH(CH₂)₆NHCOCH₃ and a molecular weight of 200.28 g/mol. This organic building block, featuring protected amine functionalities, is primarily utilised in chemical synthesis. A notable application includes its use as an inducing agent in the isolation of mononuclear cells from peripheral blood samples through Ficoll-Hypaque gradient separation.
- IUPAC
- N,N′-Diacetyl-1,6-hexanediamine,HMBA
- Synonyms
- CH3CONH(CH2)6NHCOCH3N,N′-Diacetyl-1,6-hexanediamineHMBA

Cell Isolation
N,N′-Hexamethylene bis(acetamide) functions as an inducing agent in the process of obtaining mononuclear cells from peripheral blood samples. This is typically achieved through Ficoll-Hypaque gradient separation techniques.
Chemical Synthesis
As a valuable organic building block, this compound serves as an intermediate in various chemical synthesis pathways. Its structure with protected amine groups makes it useful for introducing specific functionalities into target molecules.
Research and Development
The compound's well-defined structure and known reactivity profile make it suitable for use in research and development laboratories exploring new synthetic routes or biochemical processes.
| Molecular weight | 200.28 |
|---|---|
| Linear formula | CH3CONH(CH2)6NHCOCH3 |
| Assay | 98% |
| Solubility | water: soluble5%, clear, colorless |
| Application | N,N′-Hexamethylene bis(acetamide) was used as an inducing agent in obtaining mononuclear cells from the peripheral blood (PB) sample by Ficoll-Hypaque gradient separation1. |
| Melting point | 128-129 °C(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is N,N′-Hexamethylene bis(acetamide) used for?
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N,N′-Hexamethylene bis(acetamide) is used as an inducing agent in obtaining mononuclear cells from peripheral blood samples and as a building block in chemical synthesis.
What are the CAS number and formula for N,N′-Hexamethylene bis(acetamide)?
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The CAS number for N,N′-Hexamethylene bis(acetamide) is 3073-59-4, and its linear formula is CH₃CONH(CH₂)₆NHCOCH₃.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies N,N′-Hexamethylene bis(acetamide) with a purity of 98%. TSS does not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
How should N,N′-Hexamethylene bis(acetamide) be handled?
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Handling requires appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Refer to the safety data sheet for comprehensive handling information.
Is N,N′-Hexamethylene bis(acetamide) a controlled substance?
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The provided data does not indicate that N,N′-Hexamethylene bis(acetamide) is a controlled substance.
How is N,N′-Hexamethylene bis(acetamide) packed and shipped?
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N,N′-Hexamethylene bis(acetamide) is packed and exported by TSS in compliance with all relevant international shipping regulations for chemical products.
How can I request a quote or sample?
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To request a quote or sample of N,N′-Hexamethylene bis(acetamide), please contact the TSS sales department through our website or direct contact details.
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